Differential transcriptome analysis in HPV-positive and HPV-negative cervical cancer cells through CRISPR knockout of miR-214

被引：3

作者：

Sen, Prakriti ^{[1
]}

Ganguly, Pooja ^{[1
]}

Kulkarni, Kirti K. ^{[2
]}

Budhwar, Roli ^{[2
]}

Ganguly, Niladri ^{[1
]}

机构：

[1] KIIT Univ, Sch Biotechnol, Canc Biol Lab, Bhubaneswar, India

[2] Bionivid Technol Pvt Ltd, Kasturi Nagar, Bengaluru, India

来源：

JOURNAL OF BIOSCIENCES | 2020年 / 45卷 / 01期

关键词：

Cervical cancer; CRISPR; Human papilloma virus; miR-214; RNA transcriptome; INVASION; PROTEIN;

D O I：

10.1007/s12038-020-00075-w

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In this study we have investigated the effects of a tumour suppressor microRNA, miR-214, on gene expression in HPV-positive (CaSki) and HPV-negative cervical cancer cells (C33A) by RNA sequencing using next generation sequencing. The HPV-positive and HPV-negative cervical cancer cells were either miR-214-knocked-out or miR-214-overexpressed. Gene expression analysis showed that a total of 904 genes were upregulated and 365 genes were downregulated between HPV-positive and HPV-negative cervical cancer cells with a fold change of +/- 2. Furthermore, 11 differentially expressed and relevant genes (TNFAIP3, RAB25, MET, CYP1B1, NDRG1, CD24, LOXL2, CD44, PMS2, LATS1andMDM1) which showed a fold change of +/- 5 were selected to confirm by real-time PCR. This study represents the first report of miR-214 on global gene expression in the context of HPV.

引用

页数：25

共 33 条

[1] MicroRNAs as novel targets and tools in cancer therapy [J].

Abba, Mohammed L. ;

Patil, Nitin ;

Leupold, Joerg H. ;

Moniuszko, Marcin ;

Utikal, Jochen ;

Niklinski, Jacek ;

Allgayer, Heike .

CANCER LETTERS, 2017, 387 :84-94

[2] Primary Screening for Cervical Cancer Based on High-Risk Human Papillomavirus (HPV) Detection and HPV 16 and HPV 18 Genotyping, in Comparison to Cytology [J].

Agorastos, Theodoros ;

Chatzistamatiou, Kimon ;

Katsamagkas, Taxiarchis ;

Koliopoulos, George ;

Daponte, Alexandros ;

Constantinidis, Theocharis ;

Constantinidis, Theodoros C. .

PLOS ONE, 2015, 10 (03)

[3]

Berdasco M, 2017, TRANSL EPIGENET SER, P211, DOI 10.1016/B978-0-12-803395-1.00009-5

[4] Translating RNA sequencing into clinical diagnostics: opportunities and challenges [J].

Byron, Sara A. ;

Van Keuren-Jensen, Kendall R. ;

Engelthaler, David M. ;

Carpten, John D. ;

Craig, David W. .

NATURE REVIEWS GENETICS, 2016, 17 (05) :257-271

[5] Cytochrome P450 1B1 inhibition suppresses tumorigenicity of prostate cancer via caspase-1 activation [J].

Chang, Inik ;

Mitsui, Yozo ;

Kim, Seul Ki ;

Sun, Ji Su ;

Jeon, Hye Sook ;

Kang, Jung Yun ;

Kang, Nam Ju ;

Fukuhara, Shinichiro ;

Gill, Ankurpreet ;

Shahryari, Varahram ;

Tabatabai, Z. Laura ;

Greene, Kirsten L. ;

Dahiya, Rajvir ;

Shin, Dong Min ;

Tanaka, Yuichiro .

ONCOTARGET, 2017, 8 (24) :39087-39100

[6] LATS1 suppresses proliferation and invasion of cervical cancer [J].

Deng, Jihong ;

Zhang, Wen ;

Liu, Shuangyue ;

An, Hongmei ;

Tan, Lu ;

Ma, Lisha .

MOLECULAR MEDICINE REPORTS, 2017, 15 (04) :1654-1660

[7] The potential role of TNFAIP3 in malignant transformation of gastric carcinoma [J].

Du, Bing ;

Liu, Mingna ;

Li, Chunhong ;

Geng, Xinyu ;

Zhang, Xu ;

Ning, Dandan ;

Liu, Meiyan .

PATHOLOGY RESEARCH AND PRACTICE, 2019, 215 (08)

[8] Essential role of miRNAs in orchestrating the biology of the tumor microenvironment [J].

Frediani, Jamie N. ;

Fabbri, Muller .

MOLECULAR CANCER, 2016, 15

[9] The biological properties of E6 and E7 oncoproteins from human papillomaviruses [J].

Ghittoni, Raffaella ;

Accardi, Rosita ;

Hasan, Uzma ;

Gheit, Tarik ;

Sylla, Bakary ;

Tommasino, Massimo .

VIRUS GENES, 2010, 40 (01) :1-13

[10] HPV testing as a screen for cervical cancer [J].

Goodman, Annekathryn .

BMJ-BRITISH MEDICAL JOURNAL, 2015, 350

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