Inhibitory Effects of Caffeic Acid Phenethyl Ester Derivatives on Replication of Hepatitis C Virus

被引：36

作者：

Shen, Hui ^{[1
]}

Yamashita, Atsuya ^{[1
]}

Nakakoshi, Masamichi ^{[2
]}

Yokoe, Hiromasa ^{[3
]}

Sudo, Masashi ^{[3
]}

Kasai, Hirotake ^{[1
]}

Tanaka, Tomohisa ^{[1
]}

Fujimoto, Yuusuke ^{[1
]}

Ikeda, Masanori ^{[4
]}

Kato, Nobuyuki ^{[4
]}

Sakamoto, Naoya ^{[5
]}

Shindo, Hiroko ^{[6
]}

Maekawa, Shinya ^{[6
]}

Enomoto, Nobuyuki ^{[6
]}

Tsubuki, Masayoshi ^{[3
]}

Moriishi, Kohji ^{[1
]}

机构：

[1] Univ Yamanashi, Grad Sch Med & Engn, Dept Microbiol, Div Med, Yamanashi, Japan

[2] Toho Univ, Fac Pharmaceut Sci, Chiba 2748510, Japan

[3] Hoshi Univ, Inst Med Chem, Tokyo 142, Japan

[4] Okayama Univ, Grad Sch Med Dent, Dept Tumor Virol, Okayama 7008530, Japan

[5] Hokkaido Univ, Grad Sch Med, Dept Gastroenterol & Hepatol, Sapporo, Hokkaido, Japan

[6] Univ Yamanashi, Fac Med, Dept Internal Med 1, Yamanashi, Japan

来源：

PLOS ONE | 2013年 / 8卷 / 12期

关键词：

IN-VITRO; ANALOGS; CAPE; SUPPRESSION; ACTIVATION; EXPRESSION; CULTURE; GENOME; POTENT;

D O I：

10.1371/journal.pone.0082299

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Caffeic acid phenethyl ester (CAPE) has been reported as a multifunctional compound. In this report, we tested the effect of CAPE and its derivatives on hepatitis C virus (HCV) replication in order to develop an effective anti-HCV compound. CAPE and CAPE derivatives exhibited anti-HCV activity against an HCV replicon cell line of genotype 1b with EC50 values in a range from 1.0 to 109.6 mu M. Analyses of chemical structure and antiviral activity suggested that the length of the n-alkyl side chain and catechol moiety are responsible for the anti-HCV activity of these compounds. Caffeic acid n-octyl ester exhibited the highest anti-HCV activity among the tested derivatives with an EC50 value of 1.0 mu M and an SI value of 63.1 by using the replicon cell line derived from genotype 1b strain Con1. Treatment with caffeic acid n-octyl ester inhibited HCV replication of genotype 2a at a similar level to that of genotype 1b irrespectively of interferon signaling. Caffeic acid n-octyl ester could synergistically enhance the anti-HCV activities of interferon-alpha 2b, daclatasvir, and VX-222, but neither telaprevir nor danoprevir. These results suggest that caffeic acid n-octyl ester is a potential candidate for novel anti-HCV chemotherapy drugs.

引用

页数：11

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