Resonance light scattering determination of uranyl based on labeled DNAzyme-gold nanoparticle system

被引:42
作者
Zhou, Bin [1 ]
Shi, Lin-Fei [1 ]
Wang, Yong-Sheng [1 ]
Yang, Hui-Xian [1 ]
Xue, Jin-Hua [1 ]
Liu, Lu [1 ]
Wang, Yong-Song [1 ]
Yin, Ji-Cheng [1 ]
Wang, Jia-Cheng [1 ]
机构
[1] Univ South China, Coll Publ Hlth, Hengyang 427001, Peoples R China
基金
中国国家自然科学基金;
关键词
Resonance light scattering; DNAzyme; Gold nanoparticles; Uranyl; PLASMA-MASS SPECTROMETRY; SPECTROPHOTOMETRIC DETERMINATION; WATER SAMPLES; ARSENAZO-III; SOLID-PHASE; ICP-MS; URANIUM; EXTRACTION; BIOSENSORS; CHITOSAN;
D O I
10.1016/j.saa.2013.03.036
中图分类号
O433 [光谱学];
学科分类号
0703 ; 070302 ;
摘要
A resonance light scattering (RLS) method has been developed using a uranyl (UO22+) specific DNAzyme and gold nanoparticles (AuNPs). In this strategy, the cleavage of the substrate strand (SDNA) of DNAzyme results in releasing a shorter duplex in the presence of UO22+, leading to the aggregation of AuNPs and the increase of RLS intensity. The response signals linearly correlated with the concentration of UO22+ over the range of 1.36 x 10(-8)-1.50 x 10(-7) mol L-1. The limit of detection (LOD) is 4.09 x 10(-9) mol L-1. The method has excellent selectivity and higher sensitivity. It could provide a promising potential for the detection of metal ions, and be benefit to extend the application of RLS method. (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码:419 / 424
页数:6
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