Knockdown of long non-coding RNA MAP3K20 antisense RNA 1 inhibits gastric cancer growth through epigenetically regulating miR-375

被引：33
作者：
Quan, Yongsheng [1 ,2 ]
Zhang, Yan [3 ]
Lin, Wei [4 ]
Shen, Zhaohua [1 ,2 ]
Wu, Shuai [1 ,2 ]
Zhu, Changxin [1 ,2 ]
Wang, Xiaoyan [1 ,2 ]
机构：
[1] Cent S Univ, Xiangya Hosp 3, Dept Gastroenterol, Tongzipo Rd 138, Changsha 410013, Hunan, Peoples R China
[2] Hunan Key Lab Nonresolving Inflammat & Canc, Changsha, Hunan, Peoples R China
[3] Cent S Univ, Xiangya Hosp 3, Dept Ultrasound, Changsha, Hunan, Peoples R China
[4] Cent S Univ, Xiangya Hosp, Dept Pathol, Changsha, Hunan, Peoples R China
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2018年 / 497卷 / 02期
关键词：
Gastric cancer; Long non-coding RNA; Prognostic factor; MLK7-AS1; miR-375; Epigenetic; MICRORNA-375; CARCINOGENESIS; EXPRESSION; RECEPTOR; PDK1;
D O I：
10.1016/j.bbrc.2018.02.072
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Emerging evidence has demonstrated that long noncoding RNAs (lncRNAs) play a critical role in tumorigenesis of gastric cancer. LncRNA MAP3K20 antisense RNA 1 (MLK7-AS1) has been identified as one of gastric cancer-specific lncRNAs. However, its precise role in gastric cancer remains unknown. In this study, we found that lncRNA MLK7-AS1 was significantly increased in gastric cancer tissues compared with in adjacent tissues. Gastric cancer patients with high MLK7-AS1 expression had a shorter survival and poorer prognosis. By loss-function assay, we demonstrated that knockdown of MLK7-AS1 inhibited cell proliferation and induced apoptosis in HGC27 and MKN-45 cells. Furthermore, we identified miR-375 as a target of MLK7-AS1. MLK7-AS1 interacted with Dnmt1 and recruited it to miR-375 pro motor, hyper-methylating miR-375 promotor and repressing miR-375 expression. Taken together, our findings demonstrate that knockdown of MLK7-AS1 by siRNA inhibits gastric cancer growth by epigenetically regulating miR-375. Thus, MLK7-AS1 may be a useful prognostic marker and therapeutic target for gastric cancer patients. (C) 2018 Elsevier Inc. All rights reserved.
引用
收藏
页码：527 / 534
页数：8
相关论文
共 19 条
[1]   Tissue distribution and functional expression of a cDNA encoding a novel mixed lineage kinase [J].
Bloem, LJ ;
Pickard, RT ;
Acton, S ;
Donoghue, M ;
Beavis, RC ;
Knierman, MD ;
Wang, XS .
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, 2001, 33 (09) :1739-1750
[2]   Cancer Statistics in China, 2015 [J].
Chen, Wanqing ;
Zheng, Rongshou ;
Baade, Peter D. ;
Zhang, Siwei ;
Zeng, Hongmei ;
Bray, Freddie ;
Jemal, Ahmedin ;
Yu, Xue Qin ;
He, Jie .
CA-A CANCER JOURNAL FOR CLINICIANS, 2016, 66 (02) :115-132
[3]   The long noncoding RNA, TINCR, functions as a competing endogenous RNA to regulate PDK1 expression by sponging miR-375 in gastric cancer [J].
Chen, Zhaoliang ;
Liu, Hong ;
Yang, Huili ;
Gao, Yukai ;
Zhang, Gongwen ;
Hu, Jiaojiao .
ONCOTARGETS AND THERAPY, 2017, 10 :3353-3361
[4]   MiR-375 frequently downregulated in gastric cancer inhibits cell proliferation by targeting JAK2 [J].
Ding, Ling ;
Xu, Yanjun ;
Zhang, Wei ;
Deng, Yujie ;
Si, Misi ;
Du, Ying ;
Yao, Haomi ;
Liu, Xuyan ;
Ke, Yuehai ;
Si, Jianmin ;
Zhou, Tianhua .
CELL RESEARCH, 2010, 20 (07) :784-793
[5]   LncRNAs: emerging biomarkers in gastric cancer [J].
Gu, Yu ;
Chen, Tianxiang ;
Li, Guangliang ;
Yu, Xiongfei ;
Lu, Yimin ;
Wang, Haohao ;
Teng, Lisong .
FUTURE ONCOLOGY, 2015, 11 (17) :2427-2441
[6]   MicroRNA-375 Is Induced in Cisplatin Nephrotoxicity to Repress Hepatocyte Nuclear Factor 1-β [J].
Hao, Jielu ;
Lou, Qiang ;
Wei, Qingqing ;
Mei, Shuqin ;
Li, Lin ;
Wu, Guangyu ;
Mi, Qing-Sheng ;
Mei, Changlin ;
Dong, Zheng .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2017, 292 (11) :4571-4582
[7]   miR-375 is involved in Hippo pathway by targeting YAP1/TEAD4-CTGF axis in gastric carcinogenesis [J].
Kang, Wei ;
Huang, Tingting ;
Zhou, Yuhang ;
Zhang, Jinglin ;
Lung, Raymond W. M. ;
Tong, Joanna H. M. ;
Chan, Anthony W. H. ;
Zhang, Bin ;
Wong, Chi Chun ;
Wu, Feng ;
Dong, Yujuan ;
Wang, Shiyan ;
Yang, Weiqin ;
Pan, Yi ;
Chak, Wing Po ;
Cheung, Alvin H. K. ;
Pang, Jesse C. S. ;
Yu, Jun ;
Cheng, Alfred S. L. ;
To, Ka Fai .
CELL DEATH & DISEASE, 2018, 9
[8]   MicroRNA-375 inhibits tumour growth and metastasis in oesophageal squamous cell carcinoma through repressing insulin-like growth factor 1 receptor [J].
Kong, Kar Lok ;
Kwong, Dora Lai Wan ;
Chan, Tim Hon-Man ;
Law, Simon Ying-Kit ;
Chen, Leilei ;
Li, Yan ;
Qin, Yan-Ru ;
Guan, Xin-Yuan .
GUT, 2012, 61 (01) :33-42
[9]   Transcriptomic Analysis of Tea Plant Responding to Drought Stress and Recovery [J].
Liu, Sheng-Chuan ;
Jin, Ji-Qiang ;
Ma, Jian-Qiang ;
Yao, Ming-Zhe ;
Ma, Chun-Lei ;
Li, Chun-Fang ;
Ding, Zhao-Tang ;
Chen, Liang .
PLOS ONE, 2016, 11 (01)
[10]   Lnc RNA HOTAIR functions as a competing endogenous RNA to regulate HER2 expression by sponging miR-331-3p in gastric cancer [J].
Liu, Xiang-hua ;
Sun, Ming ;
Nie, Feng-qi ;
Ge, Ying-bin ;
Zhang, Er-bao ;
Yin, Dan-dan ;
Kong, Rong ;
Xia, Rui ;
Lu, Kai-hua ;
Li, Jin-hai ;
De, Wei ;
Wang, Ke-ming ;
Wang, Zhao-xia .
MOLECULAR CANCER, 2014, 13
12