Plasmid diversity in arctic strains of Psychrobacter spp.

被引：24

作者：

Dziewit, Lukasz ^{[1
]}

Cegielski, Adrian ^{[1
]}

Romaniuk, Krzysztof ^{[1
]}

Uhrynowski, Witold ^{[1
]}

Szych, Antoni ^{[1
]}

Niesiobedzki, Pawel ^{[1
]}

Zmuda-Baranowska, Magdalena J. ^{[2
]}

Zdanowski, Marek K. ^{[2
]}

Bartosik, Dariusz ^{[1
]}

机构：

[1] Univ Warsaw, Dept Bacterial Genet, Inst Microbiol, Fac Biol, PL-02096 Warsaw, Poland

[2] Polish Acad Sci, Inst Biochem & Biophys, Dept Antarctic Biol, PL-02141 Warsaw, Poland

来源：

EXTREMOPHILES | 2013年 / 17卷 / 03期

关键词：

Psychrobacter; Plasmid; Host range; Mobilization for conjugal transfer; ahpC; RESTRICTION-MODIFICATION SYSTEMS; GRAM-NEGATIVE BACTERIA; HOST-RANGE PLASMID; GENOME SEQUENCE; MOLECULAR CHARACTERIZATION; SP-NOV; MOBILIZATION; DNA; RESISTANCE; VECTORS;

D O I：

10.1007/s00792-013-0521-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Six strains of Psychrobacter spp. isolated from guano of little auks collected on Spitsbergen island (Arctic) carried nine plasmids that were fully sequenced. These replicons (ranging in size from 2917 to 14924 bp) contained either repA (ColE2-type) or repB (iteron-type) replication systems of a relatively narrow host range, limited to Psychrobacter spp. All but one of the plasmids carried predicted mobilization for conjugal transfer systems, encoding relaxases of the MOBQ, MOBV or MOBP families. The plasmids also contained diverse additional genetic load, including a type II restriction-modification system and a gene encoding a putative subunit C of alkyl hydroperoxide reductase (AhpC)-an antioxidant enzyme and major scavenger of reactive oxygen species. Detailed comparative sequence analyses, extended to all plasmids identified so far in psychrophilic bacteria, distinguished groups of the most ubiquitous replicons, which play a key role in horizontal gene transfer in cold environments.

引用

页码：433 / 444

页数：12

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