Simultaneous determination of plasma lopinavir and ritonavir by chemometrics-assisted spectrophotometry and comparison with HPLC method

被引：1
|
作者：
Jarusintanakorn, Salinthip [1 ]
Sripha, Kittisak [1 ]
Phechkrajang, Chutima Matayatsuk [1 ]
Wilairat, Prapin [2 ]
机构：
[1] Mahidol Univ, Fac Pharm, Dept Pharmaceut Chem, Bangkok 10400, Thailand
[2] Mahidol Univ, Natl Doping Control Ctr, Bangkok 10400, Thailand
来源：
MAEJO INTERNATIONAL JOURNAL OF SCIENCE AND TECHNOLOGY | 2013年 / 7卷 / 02期
关键词：
protease inhibitor; lopinavir; ritonavir; chemometrics; PLS-1; PERFORMANCE LIQUID-CHROMATOGRAPHY; HIV PROTEASE INHIBITORS; REVERSE-TRANSCRIPTASE INHIBITORS; SAQUINAVIR; QUANTIFICATION; NELFINAVIR; AMPRENAVIR; ATAZANAVIR; INDINAVIR; DRUGS;
D O I：
暂无
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Chemometrics-assisted spectrophotometry for the determination of two protease inhibitors, lopinavir and ritonavir, in plasma was evaluated. A set of calibration mixtures (calibration set) was designed according to central composite design. The UV spectra obtained from the calibration set were subjected to partial least square regression (PLS-1) to construct the prediction models for lopinavir and ritonavir in unknown samples, which were then validated in a randomly selected set of synthetic mixtures of the drugs. An optimum model was obtained in the wavelength ranges of 215-249 nm and 240-279 nm with principal components 8 for both lopinavir and ritonavir respectively. The prediction models were used to analyse the two drugs in plasma and the results were compared with those obtained by high-performance liquid chromatography (HPLC). The PLS-1 model and the HPLC method were found to be comparable.
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页码：248 / 257
页数：10
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