SIMULTANEOUS DETERMINATION OF OFLOXACIN AND CEFIXIME IN TABLET FORMULATION USING CAPILLARY ELECTROPHORESIS

In the current study, the first capillary electrophoresis (CE) method for the simultaneous determination of ofloxacin (OFL) and cefixime (CEF) in their combined and single dosage forms was reported. Successful separation was achieved using 31.2cm longx50 mu m I.D. fused-silica capillary, 10mM phosphate electrolyte at pH 10, injection time 10.0s, voltage 25kV and column temperature 25 degrees C. A photo diode array detector was used at wavelength 254nm. The method was validated and accuracy, precision, linearity, and limits of detection and quantitation were examined. An excellent linearity was obtained at a wide concentration range (5-200 mu g/mL). The method is also sensitive enough for pharmaceutical analysis. The limit of detection for OFL and CEF was 0.12 mu g/mL and 0.21 mu g/mL, respectively. For method application, acceptable recovery (100.2-105.3%) was obtained for all examined samples. The method has the advantages of CE over HPLC with respect to rapidity, simplicity in reagents, and sample preparation as well as saving in reagents.