A paper-based electroelution system for protein recovery from stained sodium dodecyl sulfate-polyacrylamide gels

被引：6

作者：

Branco, Alan T. ^{[1
]}

Ferreira, Beatriz dos S. ^{[1
]}

de Souza Filho, Goncalo A. ^{[1
]}

机构：

[1] Univ Estadual Norte Fluminense, Lab Biotecn, Ctr Biociencias & Biotecnol, BR-28015603 Campos Dos Goytacazes, RJ, Brazil

来源：

ANALYTICAL BIOCHEMISTRY | 2008年 / 381卷 / 02期

关键词：

D O I：

10.1016/j.ab.2008.03.025

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Electroelution is a widely used methodology for protein purification. In this study, a practical and low-cost system for protein electroeluction from stained polyacrylamide gels was developed. For this, a horizontal protein electroelution cuve was constructed with glass plates, 1.5 ml capacity microcentrifuge tubes, and dialysis membrane. Analyses of the system efficiency showed high protein recovery from non-fixed and fixed sodium dodecyl sulfate (SDS)-polyacrylamide gels. (C) 2008 Elsevier Inc. All rights reserved.

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收藏

页码：267 / 269

页数：3

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