Probing in cell protein structural changes with time-resolved X-ray scattering

被引：9
|
作者：
Spilotros, Alessandro [1 ]
Levantino, Matteo [1 ]
Schiro, Giorgio [1 ]
Cammarata, Marco [2 ]
Wulff, Michael [3 ]
Cupane, Antonio [1 ]
机构：
[1] Univ Palermo, Dept Phys, I-90123 Palermo, Italy
[2] Inst Phys Rennes, CNRS, F-35042 Rennes, France
[3] European Synchrotron Radiat Facil, F-38043 Grenoble, France
来源：
SOFT MATTER | 2012年 / 8卷 / 24期
关键词：
RED-BLOOD-CELLS; NMR-SPECTROSCOPY; LIVING CELLS; DYNAMICS; HEMOGLOBIN; RESOLUTION; ERYTHROCYTES;
D O I：
10.1039/c2sm25676b
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
Investigating protein structural changes inside the cell is a major goal in molecular biology. Here we show that time-resolved wide-angle X-ray scattering is a valuable tool for this purpose. Hemoglobin has been chosen as a model system and its tertiary and quaternary conformational changes following laser flash-photolysis have been tracked in intact red blood cells with nanosecond time resolution.
引用
收藏
页码：6434 / 6437
页数：4
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