LincRNA-uc002yug.2 involves in alternative splicing of RUNX1 and serves as a predictor for esophageal cancer and prognosis

被引：67

作者：

Wu, H. ^{[1
]}

Zheng, J. ^{[1
]}

Deng, J. ^{[1
]}

Zhang, L. ^{[2
]}

Li, N. ^{[1
]}

Li, W. ^{[1
]}

Li, F. ^{[1
]}

Lu, J. ^{[3
]}

Zhou, Y. ^{[1
]}

机构：

[1] Soochow Univ, Coll Med, Dept Genet, Suzhou 215123, Peoples R China

[2] Soochow Univ, Affiliated Hosp 2, Dept Radiotherapy & Oncol, Suzhou 215123, Peoples R China

[3] Guangzhou Med Univ, State Key Lab Resp Dis, Inst Chem Carcinogenesis, Guangzhou, Guangdong, Peoples R China

来源：

ONCOGENE | 2015年 / 34卷 / 36期

关键词：

GENOME-WIDE ASSOCIATION; SQUAMOUS-CELL CARCINOMA; LONG NONCODING RNA; SUSCEPTIBILITY LOCI; LUNG-CANCER; GENE; CHINESE; PROLIFERATION; TRANSCRIPTION; METASTASIS;

D O I：

10.1038/onc.2014.400

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Long intergenic noncoding RNAs (lincRNAs) have critical regulatory roles in cancer biology; however, the contributions of lincRNAs to esophageal squamous cell carcinoma (ESCC) have been infrequently explored. The aim of this study was to explore the contribution of lincRNAs, located at ESCC susceptibility loci identified by genome-wide association studies, to the risk and prognosis of ESCC. The associations between lincRNAs and the risk and prognosis of ESCC were analyzed in 358 diagnosed patients from eastern China, and the findings were validated in 326 additional patients from southern China. Functional relevance of lincRNAs was further examined by biochemical assays. We found that lincRNA-uc002yug.2 was commonly overexpressed in ESCC compared with paired peritumoral tissue in eastern and southern Chinese populations. The expression levels of lincRNA-uc002yug.2 in ESCC might be a prognostic factor for survival. Moreover, lincRNA-uc002yug.2 promoted a combination of RUNX1 and alternative splicing (AS) factors in the nucleus to produce more RUNX1 alpha, the short isoform and inhibitor of RUNX1, and reduce CEBP alpha (CCAAT/enhancer-binding protein-alpha) gene expression, thereby promoting ESCC progression. These results indicated that lincRNA-uc002yug.2 might involve in AS of RUNX1/AML1 and serve as a predictor for esophageal cancer and prognosis.

引用

页码：4723 / 4734

页数：12

共 45 条

[1] A shared susceptibility locus in PLCE1 at 10q23 for gastric adenocarcinoma and esophageal squamous cell carcinoma [J].

Abnet, Christian C. ;

Freedman, Neal D. ;

Hu, Nan ;

Wang, Zhaoming ;

Yu, Kai ;

Shu, Xiao-Ou ;

Yuan, Jian-Min ;

Zheng, Wei ;

Dawsey, Sanford M. ;

Dong, Linda M. ;

Lee, Maxwell P. ;

Ding, Ti ;

Qiao, You-Lin ;

Gao, Yu-Tang ;

Koh, Woon-Puay ;

Xiang, Yong-Bing ;

Tang, Ze-Zhong ;

Fan, Jin-Hu ;

Wang, Chaoyu ;

Wheeler, William ;

Gail, Mitchell H. ;

Yeager, Meredith ;

Yuenger, Jeff ;

Hutchinson, Amy ;

Jacobs, Kevin B. ;

Giffen, Carol A. ;

Burdett, Laurie ;

Fraumeni, Joseph F., Jr. ;

Tucker, Margaret A. ;

Chow, Wong-Ho ;

Goldstein, Alisa M. ;

Chanock, Stephen J. ;

Taylor, Philip R. .

NATURE GENETICS, 2010, 42 (09) :764-U51

[2] Identification and analysis of functional elements in 1% of the human genome by the ENCODE pilot project [J].

Birney, Ewan ;

Stamatoyannopoulos, John A. ;

Dutta, Anindya ;

Guigo, Roderic ;

Gingeras, Thomas R. ;

Margulies, Elliott H. ;

Weng, Zhiping ;

Snyder, Michael ;

Dermitzakis, Emmanouil T. ;

Stamatoyannopoulos, John A. ;

Thurman, Robert E. ;

Kuehn, Michael S. ;

Taylor, Christopher M. ;

Neph, Shane ;

Koch, Christoph M. ;

Asthana, Saurabh ;

Malhotra, Ankit ;

Adzhubei, Ivan ;

Greenbaum, Jason A. ;

Andrews, Robert M. ;

Flicek, Paul ;

Boyle, Patrick J. ;

Cao, Hua ;

Carter, Nigel P. ;

Clelland, Gayle K. ;

Davis, Sean ;

Day, Nathan ;

Dhami, Pawandeep ;

Dillon, Shane C. ;

Dorschner, Michael O. ;

Fiegler, Heike ;

Giresi, Paul G. ;

Goldy, Jeff ;

Hawrylycz, Michael ;

Haydock, Andrew ;

Humbert, Richard ;

James, Keith D. ;

Johnson, Brett E. ;

Johnson, Ericka M. ;

Frum, Tristan T. ;

Rosenzweig, Elizabeth R. ;

Karnani, Neerja ;

Lee, Kirsten ;

Lefebvre, Gregory C. ;

Navas, Patrick A. ;

Neri, Fidencio ;

Parker, Stephen C. J. ;

Sabo, Peter J. ;

Sandstrom, Richard ;

Shafer, Anthony .

NATURE, 2007, 447 (7146) :799-816

[3] Mechanisms of alternative splicing regulation: insights from molecular and genomics approaches [J].

Chen, Mo ;

Manley, James L. .

NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2009, 10 (11) :741-754

[4] The RUNX family in breast cancer: relationships with estrogen signaling [J].

Chimge, N-O ;

Frenkel, B. .

ONCOGENE, 2013, 32 (17) :2121-2130

[5] Functional Variants in ADH1B and ALDH2 Coupled With Alcohol and Smoking Synergistically Enhance Esophageal Cancer Risk [J].

Cui, Ri ;

Kamatani, Yoichiro ;

Takahashi, Atsushi ;

Usami, Masayuki ;

Hosono, Naoya ;

Kawaguchi, Takahisa ;

Tsunoda, Tatsuhiko ;

Kamatani, Naoyuki ;

Kubo, Michiaki ;

Nakamura, Yusuke ;

Matsuda, Koichi .

GASTROENTEROLOGY, 2009, 137 (05) :1768-1775

[6]

Dinger Marcel E., 2009, Briefings in Functional Genomics & Proteomics, V8, P407, DOI 10.1093/bfgp/elp038

[7] Gastrointestinal Adenocarcinomas of the Esophagus, Stomach, and Colon Exhibit Distinct Patterns of Genome Instability and Oncogenesis [J].

Dulak, Austin M. ;

Schumacher, Steven E. ;

van Lieshout, Jasper ;

Imamura, Yu ;

Fox, Cameron ;

Shim, Byoungyong ;

Ramos, Alex H. ;

Saksena, Gordon ;

Baca, Sylvan C. ;

Baselga, Jose ;

Tabernero, Josep ;

Barretina, Jordi ;

Enzinger, Peter C. ;

Corso, Giovanni ;

Roviello, Franco ;

Lin, Lin ;

Bandla, Santhoshi ;

Luketich, James D. ;

Pennathur, Arjun ;

Meyerson, Matthew ;

Ogino, Shuji ;

Shivdasani, Ramesh A. ;

Beer, David G. ;

Godfrey, Tony E. ;

Beroukhim, Rameen ;

Bass, Adam J. .

CANCER RESEARCH, 2012, 72 (17) :4383-4393

[8] The functional role of long non-coding RNA in human carcinomas [J].

Gibb, Ewan A. ;

Brown, Carolyn J. ;

Lam, Wan L. .

MOLECULAR CANCER, 2011, 10

[9] RARα-PLZF oncogene inhibits C/EBPα function in myeloid cells [J].

Girard, Nathalie ;

Tremblay, Mathieu ;

Humbert, Magali ;

Grondin, Benoit ;

Haman, Andre ;

Labrecque, Jean ;

Chen, Bing ;

Chen, Zhu ;

Chen, Sai-Juan ;

Trang Hoang .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2013, 110 (33) :13522-13527

[10] Runx1 deletion or dominant inhibition reduces Cebpa transcription via conserved promoter and distal enhancer sites to favor monopoiesis over granulopoiesis [J].

Guo, Hong ;

Ma, Ou ;

Speck, Nancy A. ;

Friedman, Alan D. .

BLOOD, 2012, 119 (19) :4408-4418

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