Thiol-ene Mediated Neoglycosylation of Collagen Patches: A Preliminary Study

被引：40

作者：

Russo, Laura ^{[1
]}

Battocchio, Chiara ^{[2
,3
]}

Secchi, Valeria ^{[2
,3
]}

Magnano, Elena ^{[4
]}

Nappini, Silvia ^{[4
]}

Taraballi, Francesca ^{[1
]}

Gabrielli, Luca ^{[1
]}

Comelli, Francesca ^{[1
]}

Papagni, Antonio ^{[5
]}

Costa, Barbara ^{[1
]}

Polzonetti, Giovanni ^{[2
,3
]}

Nicotra, Francesco ^{[1
]}

Natalello, Antonino ^{[1
]}

Doglia, Silvia M. ^{[1
]}

Cipolla, Laura ^{[1
]}

机构：

[1] Univ Milano Bicocca, Dept Biotechnolgy & Biosci, I-20126 Milan, Italy

[2] Univ Roma Tre, CNISM, INSTM, Dept Sci, I-00146 Rome, Italy

[3] Univ Roma Tre, CISDiC, I-00146 Rome, Italy

[4] CNR, IOM CNR Lab TASC, I-34149 Trieste, Italy

[5] Univ Milano Bicocca, Dept Mat Sci, I-20125 Milan, Italy

来源：

LANGMUIR | 2014年 / 30卷 / 05期

关键词：

GLYCOSYLATION; CARTILAGE; CARBOHYDRATE; DIFFERENTIATION; SURFACES; PEPTIDE; EPITOPE; SWEET;

D O I：

10.1021/la404310p

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Despite the relevance of carbohydrates as cues in eliciting specific biological responses, the covalent surface modification of collagen-based matrices with small carbohydrate epitopes has been scarcely investigated. We report thereby the development of an efficient procedure for the chemoselective neoglycosylation of collagen matrices (patches) via a thiol-ene approach, between alkene-derived mono-saccharides and the thiol-functionalized material surface. Synchrotron radiation-induced X-ray photoelectron spectroscopy (SR-XPS), Fourier transform-infrared (FT-IR), and enzyme-linked lectin assay (ELLA) confirmed the effectiveness of the collagen neoglycosylation. Preliminary biological evaluation in osteoarthritic models is reported. The proposed methodology can be extended to any thiolated surface for the development of smart biomaterials for innovative approaches in regenerative medicine.

引用

页码：1336 / 1342

页数：7

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