Preparation and Characterization of Glucose Biosensors Using Self-Assembled Monolayers of Alkanethiols

被引：2

作者：

Lee, Wen-Lung ^{[1
]}

Lai, Shih-Ming ^{[1
]}

机构：

[1] Natl Yunlin Univ Sci & Technol, Dept Chem & Mat Engn, Touliu 640, Yunlin, Taiwan

来源：

SENSOR LETTERS | 2008年 / 6卷 / 06期

关键词：

Self-Assembled Monolayers; Alkanethiols; Glucose Biosensors; Chronoamperometry;

D O I：

10.1166/sl.2008.550

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Glucose biosensors were prepared by immobilizing glucose oxidase on gold electrodes modified with a self-assembled monolayer (SAM) of 3-mercaptopropionic acid (MPA) in this study. The important parameters in this fabrication process were investigated and the optimal fabrication conditions were selected. Using the glucose biosensors manufactured by the optimal procedure, the glucose concentration measurements were performed by chronoamperometry, where the optimal operating potential and time were selected. Also, the effect of mediator concentration on the sensitivity of the SAMs based glucose biosensors was investigated.

引用

页码：1005 / 1009

页数：5

共 11 条

[1] A FULLY ACTIVE MONOLAYER ENZYME ELECTRODE DERIVATIZED BY ANTIGEN-ANTIBODY ATTACHMENT [J].

BOURDILLON, C ;

DEMAILLE, C ;

GUERIS, J ;

MOIROUX, J ;

SAVEANT, JM .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1993, 115 (26) :12264-12269

[2] Desorption of spontaneously adsorbed and electrochemically readsorbed 2-mercaptoethanesulfonate on Au(111) [J].

Calvente, JJ ;

Kovacova, Z ;

Sanchez, MD ;

Andreu, R ;

Fawcett, WR .

LANGMUIR, 1996, 12 (23) :5696-5703

[3] Self-assembled monolayers as a tunable platform for biosensor applications [J].

Chaki, NK ;

Vijayamohanan, K .

BIOSENSORS & BIOELECTRONICS, 2002, 17 (1-2) :1-12

[4] Self-assembled monolayers as the coating in a quartz piezoelectric crystal immunosensor to detect salmonella in aqueous solution [J].

Fung, YS ;

Wong, YY .

ANALYTICAL CHEMISTRY, 2001, 73 (21) :5302-5309

[5] The application of alkanethiol self-assembled monolayers to enzyme electrodes [J].

Gooding, JJ ;

Hibbert, DB .

TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 1999, 18 (08) :525-533

[6] Parameters important in fabricating enzyme electrodes using self-assembled monolayers of alkanethiols [J].

Gooding, JJ ;

Erokhin, P ;

Losic, D ;

Yang, WR ;

Policarpio, V ;

Liu, JQ ;

Ho, FM ;

Situmorang, M ;

Hibbert, DB ;

Shapter, JG .

ANALYTICAL SCIENCES, 2001, 17 (01) :3-9

[7] In situ scanning tunneling microscopy observation of self-assembled monolayers of 3-mercaptopropionic acid on Au(111) in perchloric acid solution [J].

Sawaguchi, T ;

Sato, Y ;

Mizutani, F .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 2001, 507 (1-2) :256-262

[8] Immobilisation of enzyme throughout a polytyramine matrix: a versatile procedure for fabricating biosensors [J].

Situmorang, M ;

Gooding, JJ ;

Hibbert, DB .

ANALYTICA CHIMICA ACTA, 1999, 394 (2-3) :211-223

[9] Multilayered construction of glucose oxidase and silica nanoparticles on Au electrodes based on layer-by-layer covalent attachment [J].

Sun, YY ;

Yan, F ;

Yang, WS ;

Sun, CQ .

BIOMATERIALS, 2006, 27 (21) :4042-4049

[10] An amperometric glucose sensor with modified Langmuir-Blodgett films [J].

Tsuji, H ;

Mitsubayashi, K .

ELECTROANALYSIS, 1997, 9 (02) :161-164

← 1 2 →