Forensic SNP Genotyping using Nanopore MinION Sequencing

被引：51

作者：

Cornelis, Senne ^{[1
]}

Gansemans, Yannick ^{[1
]}

Deleye, Lieselot ^{[1
]}

Deforce, Dieter ^{[1
]}

Van Nieuwerburgh, Filip ^{[1
]}

机构：

[1] Univ Ghent, Lab Pharmaceut Biotechnol, Ghent, Belgium

来源：

SCIENTIFIC REPORTS | 2017年 / 7卷

关键词：

SINGLE NUCLEOTIDE POLYMORPHISMS; IDENTIFICATION; ASSAY;

D O I：

10.1038/srep41759

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

One of the latest developments in next generation sequencing is the Oxford Nanopore Technologies' (ONT) MinION nanopore sequencer. We studied the applicability of this system to perform forensic genotyping of the forensic female DNA standard 9947 A using the 52 SNP-plex assay developed by the SNPforID consortium. All but one of the loci were correctly genotyped. Several SNP loci were identified as problematic for correct and robust genotyping using nanopore sequencing. All these loci contained homopolymers in the sequence flanking the forensic SNP and most of them were already reported as problematic in studies using other sequencing technologies. When these problematic loci are avoided, correct forensic genotyping using nanopore sequencing is technically feasible.

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