Protective effect of chitooligosaccharides against cyclophosphamide-induced immunosuppression in mice

被引：89
作者：
Mei, Yu-xia [1 ]
Chen, Hong-xia [1 ]
Zhang, Jun [1 ]
Zhang, Xue-dan [1 ]
Liang, Yun-xiang [1 ]
机构：
[1] Huazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China
来源：
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2013年 / 62卷
关键词：
Chitooligosaccharides; Immunoenhancement; Immunosuppression; WATER-SOLUBLE CHITOSAN; IN-VITRO; POLYSACCHARIDES; MACROPHAGES; CYTOKINES; RESPONSES; VIVO; OLIGOCHITOSAN; ACTIVATION; INDUCTION;
D O I：
10.1016/j.ijbiomac.2013.09.038
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Chitooligosaccharides (COS) display a variety of important biological activities, including antimicrobial, antitumor, anti-inflammatory, and immunoenhancing. In the present study, a COS sample (degree of polymerization 4-11, analyzed by FTIR and MALDI-TOF-MS) prepared by hydrolysis with a recombinant chitosanase was orally administered to mice for evaluation of its effect on cyclophosphamide (Cy)-induced immunosuppression. Thymus and spleen indices, delayed-type hypersensitivity (DTH) reaction, macrophage phagocytosis, and certain enzyme activities were significantly higher in mice treated with COS + Cy than in mice treated with Cy alone. ELISA experiments showed that COS treatment enhanced production of the cytokines IL-2, IL-12, and IFN-gamma but decreased production of IL-10 in sera of Cy-treated mice. The well-defined COS product studied displayed strong immunoenhancing activity and a protective effect against Cy-induced immunosuppression. COS-derived products should be further investigated for possible immunostimulatory applications in the food and pharmaceutical industries. (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码：330 / 335
页数：6
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