Mechanistic studies on the role of TGF-β1 in angiogenesis through EndMT

被引：10
作者：
Cao, Sheng-Jun [1 ]
Hong, Lei [2 ]
Li, Xiao-Qiang [3 ]
机构：
[1] Taizhou Second Peoples Hosp, Dept Vasc Surg, Taizhou, Jiangsu, Peoples R China
[2] USTC, Dept Affiliated Hosp 1, Hefei, Anhui, Peoples R China
[3] Nanjing Drum Tower Hosp, Dept Vasc Surg, 321 Zhongshan Rd, Nanjing 215006, Jiangsu, Peoples R China
来源：
VASCULAR | 2021年 / 29卷 / 03期
关键词：
Endothelial-to-mesenchymal transition; vascular endothelial cell growth factor; umbilical vein endothelial cells; angiogenesis; mechanism research; TO-MESENCHYMAL TRANSITION; CELLS;
D O I：
10.1177/1708538120953668
中图分类号：
R6 [外科学];
学科分类号：
1002 ; 100210 ;
摘要：
Objective This study aims to investigate the mechanism of transforming growth factor-beta 1 (TGF-beta 1) in promoting angiogenesis through endothelial-to-mesenchymal transition (EndMT). Methods The mesenchymal transition of human umbilical vein endothelial cells (HUVECs) was induced by TGF-beta 1. The angiogenesis, migration, and proliferation of HUVECs undergoing EndMT were examined by tube formation assay, scratch assay, Transwell assay, and CCK-8 assay. Results The outcomes revealed that EndMT promoted angiogenesis, migration, and proliferation of HUVECs and the secretion of the vascular endothelial growth factor (VEGF) of HUVECs. Phosphorylated AKT (p-AKT) increased in EndMT by inhibiting the mitigation of angiogenesis. Conclusion EndMT induces angiogenesis by promoting the secretion of VEGF, and p-AKT participates in this regulation.
引用
收藏
页码：442 / 450
页数：9
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