Assessment of female gamete quality in the Pacific oyster Crassostrea gigas

被引:10
作者
Ramirez, MEV
Le Pennec, M
Dorange, G
Devauchelle, N
Nonnotte, G
机构
[1] IUEM, Lab Bioflux, F-29280 Plouzane, France
[2] Univ Bretagne Occidentale, Unite Culture Cellulaire, F-29285 Brest, France
[3] IFREMER, Ctr Brest, F-29280 Plouzane, France
[4] Univ Bretagne Occidentale, Lab Physiol Syst Integres, F-29285 Brest, France
关键词
oocytes; embryos; quality indicators; Crassostrea gigas;
D O I
10.1080/07924259.1999.9652680
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The possible relationship between certain oocyte and embryo characteristics and larvae viability was investigated with reference to the following aspects: (1) morphological - oocyte diameter and shape; (2) cytological - overall ultrastructure and membrane integrity; (3) biochemical - content of lipids, proteins and carbohydrates; and (4) physiological - respiration. The rate of survival and incidence of abnormality were estimated 24h after fertilization, The first results showed that 80-90% of oocytes were cytologically viable before fertilization, Eighty to 90% of oocytes are apparently viable before fertilization on the basis of staining with Trypan blue, but this parameter shows little correlation with larval viability. However, Trypan blue staining is of value in allowing the recognition of oocytes with damaged membranes, Respiration was measured for unfertilized oocytes 5 min after stripping, after 6 h, and for 3-h embryos. Positive correlations were found between the O-2-consumption of embryos and both the rate of fertilization and the hatching rate of 24-h larvae, In contrast, no correlation was found between hatching parameters and the O-2-consumption of unfertilized oocytes. These results suggest that embryos possess quality indicators, relating to metabolic characteristics, which can be quantified more easily than those of oocytes.
引用
收藏
页码:73 / 78
页数:6
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