CD44v6 Monoclonal Antibody-Conjugated Gold Nanostars for Targeted Photoacoustic Imaging and Plasmonic Photothermal Therapy of Gastric Cancer Stem-like Cells

被引：128
作者：
Liang, Shujing [1 ,2 ]
Li, Chao [2 ]
Zhang, Chunlei [2 ]
Chen, Yunsheng [2 ]
Xu, Liang [1 ]
Bao, Chenchen [2 ]
Wang, Xiaoyong [3 ]
Liu, Gang [3 ]
Zhang, Fengchun [1 ,4 ]
Cui, Daxiang [2 ]
机构：
[1] Shanghai Jiao Tong Univ, Sch Med, Rui Jin Hosp, Shanghai 200025, Peoples R China
[2] Shanghai Jiao Tong Univ, Inst Nano Biomed & Engn, Key Lab Thin Film & Microfabricat,Minist Educ, Dept Instrument Sci & Engn,Res Inst Translat Med, Shanghai 200240, Peoples R China
[3] Xiamen Univ, Ctr Mol Imaging & Translat Med, Xiamen 361102, Peoples R China
[4] Shanghai Jiao Tong Univ, Suzhou Kowloon Hosp, Sch Med, Shanghai, Peoples R China
来源：
THERANOSTICS | 2015年 / 5卷 / 09期
关键词：
gold nanostars; CD44v6 monoclonal antibody; gastric cancer stem cells (GCSCs); photoacoustic imaging; photothermal therapy; IN-VIVO; IDENTIFICATION; RESISTANCE; LINE; NANOPARTICLES; MICROSCOPY; STRATEGIES; INDUCTION; NANORODS;
D O I：
10.7150/thno.11632
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Developing safe and effective nanoprobes for targeted imaging and selective therapy of gastric cancer stem cells (GCSCs) has become one of the most promising anticancer strategies. Herein, gold nanostars-based PEGylated multifunctional nanoprobes were prepared with conjugated CD44v6 monoclonal antibodies (CD44v6-GNS) as the targeting ligands. It was observed that the prepared nanoprobes had high affinity towards GCSC spheroid colonies and destroyed them completely with a low power density upon near-infrared (NIR) laser treatment (790 nm, 1.5 W/cm(2), 5 min) in vitro experiment. Orthotopic and subcutaneous xenografted nude mice models of human gastric cancer were established. Subsequently, biodistribution and photothermal therapeutic effects after being intravenously injected with the prepared nanoprobes were assessed. Photoacoustic imaging revealed that CD44v6-GNS nanoprobes could target the gastric cancer vascular system actively at 4 h post-injection, while the probes inhibited tumor growth remarkably upon NIR laser irradiation, and even extended survivability of the gastric cancer-bearing mice. The CD44v6-GNS nanoprobes exhibited great potential for applications of gastric cancer targeted imaging and photothermal therapy in the near future.
引用
收藏
页码：970 / 984
页数：15
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