Recent Advancements in the LC- and GC-Based Analysis of Malondialdehyde (MDA): A Brief Overview

被引：149
作者：
Giera, Martin [1 ,2 ]
Lingeman, Henk [2 ]
Niessen, Wilfried M. A. [2 ]
机构：
[1] LUMC, Biomol Mass Spectrometry Unit, NL-2300 RC Leiden, Netherlands
[2] Vrije Univ Amsterdam, NL-1081 HV Amsterdam, Netherlands
来源：
CHROMATOGRAPHIA | 2012年 / 75卷 / 9-10期
关键词：
Malondialdehyde (MDA); Derivatization; LC-MS(MS); Biomarker; PERFORMANCE LIQUID-CHROMATOGRAPHY; LIPID-PEROXIDATION PRODUCTS; ELECTRON-CAPTURE DETECTION; HUMAN PLASMA; GAS-CHROMATOGRAPHY; FLUORESCENCE DETECTION; BIOLOGICAL SAMPLES; OXIDATIVE STRESS; MASS-SPECTROMETRY; DERIVATIZATION;
D O I：
10.1007/s10337-012-2237-1
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Malondialdehyde (MDA) is an end-product of lipid peroxidation and a side product of thromboxane A(2) synthesis. Moreover, it is not only a frequently measured biomarker of oxidative stress, but its high reactivity and toxicity underline the fact that this molecule is more than "just" a biomarker. Additionally, MDA was proven to be a mutagenic substance. Having said this, it is evident that there is a major interest in the highly selective and sensitive analysis of this molecule in various matrices. In this review, we will provide a brief overview of the most recent developments and techniques for the liquid chromatography (LC) and gas chromatography (GC)-based analysis of MDA in different matrices. While the 2-thiobarbituric acid assay still is the most prominent methodology for determining MDA, several advanced techniques have evolved, including GC-MS(MS), LC-MS(MS) as well as several derivatization-based strategies.
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收藏
页码：433 / 440
页数：8
相关论文
共 45 条
[1]   Rapid, fluorimetric-liquid chromatographic determination of malondialdehyde in biological samples [J].
Agarwal, R ;
Chase, SD .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2002, 775 (01) :121-126
[2]   Measurement of malondialdehyde levels in food by high-performance liquid chromatography with fluorometric detection [J].
Bergamo, P ;
Fedele, E ;
Balestrieri, M ;
Abrescia, P ;
Ferrara, L .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1998, 46 (06) :2171-2176
[3]   DNA adducts with lipid peroxidation products [J].
Blair, Ian A. .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2008, 283 (23) :15545-15549
[4]   An inter-laboratory validation of methods of lipid peroxidation measurement in UVA-treated human plasma samples [J].
Breusing, Nicolle ;
Grune, Tilman ;
Andrisic, Luka ;
Atalay, Mustafa ;
Bartosz, Grzegorz ;
Biasi, Fiorella ;
Borovic, Suzana ;
Bravo, Laura ;
Casals, Isidre ;
Casillas, Rosario ;
Dinischiotu, Anca ;
Drzewinska, Joanna ;
Faber, Heidemarie ;
Fauzi, Norsyahida Mohd ;
Gajewska, Agnieszka ;
Gambini, Juan ;
Gradinaru, Daniela ;
Kokkola, Tarja ;
Lojek, Antonin ;
Luczaj, Wojciech ;
Margina, Denisa ;
Mascia, Cinzia ;
Mateos, Raquel ;
Meinitzer, Andreas ;
Teresa Mitjavila, Maria ;
Mrakovcic, Lidija ;
Munteanu, Maria Cristina ;
Podborska, Martina ;
Poli, Giuseppe ;
Sicinska, Paulina ;
Skrzydlewska, Elzbieta ;
Vina, Jose ;
Wiswedel, Ingrid ;
Zarkovic, Neven ;
Zelzer, Sieglinde ;
Spickett, Corinne M. .
FREE RADICAL RESEARCH, 2010, 44 (10) :1203-1215
[5]   Role of Radicals in the Lipid Peroxidation Products of Commercial Infant Milk Formula [J].
Burg, Ariela ;
Silberstein, Tali ;
Yardeni, Guy ;
Tavor, Dorith ;
Blumenfeld, Jeanine ;
Zilbermann, Israel ;
Saphier, Oshra .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2010, 58 (04) :2347-2350
[6]   Free and total malondialdehyde assessment in biological matrices by gas chromatography mass spectrometry: What is needed for an accurate detection [J].
Cighetti, G ;
Debiasi, S ;
Paroni, R ;
Allevi, P .
ANALYTICAL BIOCHEMISTRY, 1999, 266 (02) :222-229
[7]   A review of recent studies on malondialdehyde as toxic molecule and biological marker of oxidative stress [J].
Del Rio, D ;
Stewart, AJ ;
Pellegrini, N .
NUTRITION METABOLISM AND CARDIOVASCULAR DISEASES, 2005, 15 (04) :316-328
[8]   Simultaneous determination of eight lipid peroxidation degradation products in urine of rats treated with carbon tetrachloride using gas chromatography with electron-capture detection [J].
DeZwart, LL ;
Venhorst, J ;
Groot, M ;
Commandeur, JNM ;
Hermanns, RCA ;
Meerman, JHM ;
VanBaar, BLM ;
Vermeulen, PE .
JOURNAL OF CHROMATOGRAPHY B, 1997, 694 (02) :277-287
[9]   Human plasma concentrations of malondialdehyde (MDA) and the F2-isoprostane 15(S)-8-iso-PGF2α may be markedly compromised by hemolysis: Evidence by GC-MS/MS and potential analytical and biological ramifications [J].
Dreissigacker, Ulrike ;
Suchy, Maria-Theresia ;
Maassen, Norbert ;
Tsikas, Dimitrios .
CLINICAL BIOCHEMISTRY, 2010, 43 (1-2) :159-167
[10]   Development of an on-line weak-cation exchange liquid chromatography-tandem mass spectrometric method for screening aldehyde products in biological matrices [J].
Eggink, Mark ;
Charret, Segolene ;
Wijtmans, Maikel ;
Lingeman, Henk ;
Kool, Jeroen ;
Niessen, Wilfried M. A. ;
Irth, Hubertus .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2009, 877 (31) :3937-3945
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