Kidney-derived proteins in urine as biomarkers of induced acute kidney injury in sheep

被引：12

作者：

Palviainen, Mari ^{[1
]}

Raekallio, Marja ^{[1
]}

Rajamaki, Minna M. ^{[1
]}

Linden, Jere ^{[2
]}

Vainio, Outi ^{[1
]}

机构：

[1] Univ Helsinki, Pharmacol & Toxicol Sect, Dept Equine & Small Anim Med, Fac Vet Med, FIN-00014 Helsinki, Finland

[2] Univ Helsinki, Dept Vet Biosci, Fac Vet Med, FIN-00014 Helsinki, Finland

来源：

VETERINARY JOURNAL | 2012年 / 193卷 / 01期

关键词：

Proteomics; Calbindin-D28k; Retinol-binding protein 4; CD1d; Urine; RETINOL-BINDING-PROTEIN; CD1D GENE; IDENTIFICATION; TRANSPORT;

D O I：

10.1016/j.tvjl.2011.10.004

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Acute kidney injury (AKI) is a life-threatening condition for which an early diagnosis is problematic. The aim of the present study was to identify kidney-derived urinary proteins specific to AKI in sheep. AKI was induced in six sheep by an overdose of ketoprofen. Six untreated sheep served as controls. Urine samples were collected for up to 24 h after drug administration and pooled according to time and treatment. Tissue samples from kidney were taken immediately after euthanasia. Urinary proteins were separated by two-dimensional gel electrophoresis (2DE) and the proteins of interest were identified by mass spectrometry. Calbindin-D28k, retinol-binding protein 4 and CD1d were identified in ketoprofen-treated sheep, but not in controls. In addition, calbindin-D28k and CD1d were localized in kidney tissues by immunohistochemical staining. These preliminary results suggest that urinary calbindin-D28k and CD1d represent potential useful biomarkers of AKI, at least in sheep. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：287 / 289

页数：3

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