Plasma functionalization procedure for antibody immobilization for SU-8 based sensor

被引：22

作者：

Grimaldi, Immacolata Angelica ^{[1
]}

Testa, Genrii ^{[1
]}

Persichetti, Gianluca ^{[1
]}

Loffredo, Fausta ^{[2
]}

Villani, Fulvia ^{[2
]}

Bernini, Romeo ^{[1
]}

机构：

[1] Natl Res Council CNR, Inst Electromagnet Monitoring Environm IREA, Naples, Italy

[2] Italian Natl Agcy New Technol, Energy & Sustainable Econ Dev ENEA, Portici Res Ctr, Ple Enrico Fermi 1, I-80055 Naples, Italy

来源：

BIOSENSORS & BIOELECTRONICS | 2016年 / 86卷

关键词：

Optical biosensor; Immunoglobulin G; Surface immobilization; Biosensing; Microring resonator; SURFACE MODIFICATION; REAL-TIME; BIOMOLECULES; SILANIZATION; PHOTORESIST; SENSITIVITY; PLATFORM; BINDING; MEMS;

D O I：

10.1016/j.bios.2016.07.090

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

In this paper, we report the study on a new protocol for the immobilization process of antigen/antibody assay on SU-8 layers by oxygen plasma treatment. Plasma treatments, at different plasma powers and for different duration times, are performed and their effects on immobilization efficiency are studied. The chemical properties and the surface morphology of SU-8 before and after the functionalization and immobilization of (IgG) are then verified by Raman spectroscopy and atomic force microscopy (AFM). An increase of the surface roughness of SU-8 layers is observed after the oxygen plasma treatment and an intensity variation of functional groups is also evidenced. To demonstrate the validity of the process the distribution of IgG immobilized on SU-8 surfaces is detected by fluorescence microscopy measurement after incubation with fluorescein isothiocyanate (FITC)-tagged anti-human IgG. An increase of the amount of the adsorbed protein of about 20% and a good repeatability on antigen/antibody distribution on the surface are detected for IgG on plasma treated substrates. Finally, label free measurements are performed by SU-8 optical ring resonators reaching detection limits of 0.86 ng cm(-2). The proposed approach offers a smart protocol for IgG immobilization on SU-8 substrate that can be easily extended to different antigen/antibody assay and polymeric materials for the realization of high performance immunosensors. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：827 / 833

页数：7

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