Seasonal bacterial community dynamics in a full-scale enhanced biological phosphorus removal plant

被引:75
作者
Flowers, Jason J. [1 ,2 ]
Cadkin, Tracey A. [2 ]
McMahon, Katherine D. [2 ,3 ]
机构
[1] Univ Washington, Dept Civil & Environm Engn, Seattle, WA 98105 USA
[2] Univ Wisconsin, Dept Civil & Environm Engn, Madison, WI 53706 USA
[3] Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA
基金
美国国家科学基金会; 美国国家卫生研究院;
关键词
Enhanced biological phosphorus removal; Candidatus Accumulibacter phosphatis; Activated sludge; POLYPHOSPHATE-ACCUMULATING ORGANISMS; CANDIDATUS-ACCUMULIBACTER; POPULATION-DYNAMICS; DIVERSITY; SYSTEM; IMMIGRATION; PERFORMANCE; MUNICIPAL;
D O I
10.1016/j.watres.2013.07.054
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Activated sludge is one of the most abundant and effective wastewater treatment process used to treat wastewater, and has been used in developed countries for nearly a century. In all that time, several hundreds of studies have explored the bacterial communities responsible for treatment, but most studies were based on a handful of samples and did not consider temporal dynamics. In this study, we used the DNA fingerprinting technique called automated ribosomal intergenic spacer region analysis (ARISA) to study bacterial community dynamics over a two-year period in two different treatment trains. We also used quantitative PCR to measure the variation of five phylogenetically-defined clades within the Accumulibacter lineage, which is a model polyphosphate accumulating organism. The total bacterial community exhibited seasonal patterns of change reminiscent of those observed in lakes and oceans. Surprisingly, all five Accumulibacter clades were present throughout the study, and the total Accumulibacter community was relatively stable. However, the abundance of each clade did fluctuate through time. Clade IIA dynamics correlated positively with temperature (rho = 0.65, p < 0.05) while Cade IA dynamics correlated negatively with temperature (rho = 0.35, p < 0.05). This relationship with temperature hints at the mechanisms that may be driving the seasonal patterns in overall bacterial community dynamics and provides further evidence for ecological differentiation among clades within the Accumulibacter lineage. This work provides a valuable baseline for activated sludge bacterial community variation. (C) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码:7019 / 7031
页数:13
相关论文
共 43 条
[1]   Multivariate dispersion as a measure of beta diversity [J].
Anderson, MJ ;
Ellingsen, KE ;
McArdle, BH .
ECOLOGY LETTERS, 2006, 9 (06) :683-693
[2]  
[Anonymous], 1983, Methods for chemical analysis of water and wastes
[3]  
[Anonymous], 1999, Standard Methods for Examination of Water and Waste Water
[4]   Which are the polyphosphate accumulating organisms in full-scale activated sludge enhanced biological phosphate removal systems in Australia? [J].
Beer, M ;
Stratton, HM ;
Griffiths, PC ;
Seviour, RJ .
JOURNAL OF APPLIED MICROBIOLOGY, 2006, 100 (02) :233-243
[5]   Influence of temperature on biological phosphorus removal: Process and molecular ecological studies [J].
Brdjanovic, D ;
Logemann, S ;
Van Loosdrecht, MCM ;
Hooijmans, CM ;
Alaerts, GJ ;
Heijnen, JJ .
WATER RESEARCH, 1998, 32 (04) :1035-1048
[6]   Denitrifying phosphorus removal: Linking the process performance with the microbial community structure [J].
Carvalho, Gilda ;
Lemos, Paulo C. ;
Oehmen, Adrian ;
Reis, Maria A. M. .
WATER RESEARCH, 2007, 41 (19) :4383-4396
[7]   NONPARAMETRIC MULTIVARIATE ANALYSES OF CHANGES IN COMMUNITY STRUCTURE [J].
CLARKE, KR .
AUSTRALIAN JOURNAL OF ECOLOGY, 1993, 18 (01) :117-143
[8]   Characterisation of the microbial 16S rDNA diversity of an aerobic phosphorus-removal ecosystem and monitoring of its transition to nitrate respiration [J].
Dabert, P ;
Sialve, B ;
Delgenès, JP ;
Moletta, R ;
Godon, JJ .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2001, 55 (04) :500-509
[9]  
Environmental Protection Agency (EPA) U.S, 1993, METH DET IN SUBST EN
[10]   Is it PAO-GAO competition or metabolic shift in EBPR system? Evidence from an experimental study [J].
Erdal, Ufuk G. ;
Erdal, Zeynep K. ;
Daigger, Glen T. ;
Randall, Clifford W. .
WATER SCIENCE AND TECHNOLOGY, 2008, 58 (06) :1329-1334