Effect of bisphenol A on pluripotency of mouse embryonic stem cells and differentiation capacity in mouse embryoid bodies

被引：39
作者：
Chen, Xiaojiao [1 ,2 ]
Xu, Bo [2 ]
Han, Xiumei [2 ]
Mao, Zhilei [2 ]
Talbot, Prue [3 ,4 ]
Chen, Minjian [2 ]
Du, Guizhen [2 ]
Chen, Aiqin [5 ]
Liu, Jiayin [5 ]
Wang, Xinru [1 ,2 ]
Xia, Yankai [1 ,2 ]
机构：
[1] Nanjing Med Univ, State Key Lab Reprod Med, Nanjing Matern & Child Hlth Hosp, Nanjing 210029, Jiangsu, Peoples R China
[2] Nanjing Med Univ, Sch Publ Hlth, Key Lab Modern Toxicol, Minist Educ, Nanjing 211166, Jiangsu, Peoples R China
[3] Univ Calif Riverside, UCR Stem Cell Ctr, Riverside, CA 92521 USA
[4] Univ Calif Riverside, Dept Cell Biol & Neurosci, Core Facil, Riverside, CA 92521 USA
[5] Nanjing Med Univ, Affiliated Hosp 1, State Key Lab Reprod Med, Clin Ctr Reprod Med, Nanjing 210029, Jiangsu, Peoples R China
来源：
TOXICOLOGY IN VITRO | 2013年 / 27卷 / 08期
关键词：
Bisphenol A; Mouse embryonic stem cells; Mouse embryoid bodies; Pluripotency; Differentiation; MicroRNA; IN-VITRO DIFFERENTIATION; SELF-RENEWAL; EXPRESSION; EXPOSURE; URINARY; MODEL; MICRORNAS; NETWORK; OCT4; GENE;
D O I：
10.1016/j.tiv.2013.09.018
中图分类号：
R99 [毒物学（毒理学）];
学科分类号：
100405 ;
摘要：
Bisphenol A (BPA) poses potential risks to reproduction and development. However, the mechanism of BPA's effects on early embryonic development is still unknown. Embryonic stem cells (ESC) and embryoid bodies (EB) provide valuable in vitro models for testing the toxic effects of environmental chemicals in early embryogenesis. In this study, mouse embryonic stem cells (mESC) were acutely exposed to BPA for 24 h, and general cytotoxicity and the effect of BPA on pluripotency were then evaluated. Meanwhile, mouse embryoid bodies (mEB) were exposed to BPA up to 6 days and their differentiation capacity was evaluated. In mESC and mEB, we found that BPA up-regulated pluripotency markers (Oct4, Sox2 and Nanog) at mRNA and/or protein levels. Moreover, BPA increased the mRNA levels of endodermal markers (Gata4,Sox17) and mesodermal markers (Sma,Desmin), and reduced the mRNA levels of ectodermal markers (Nestin,Fgf5) in mEB. Furthermore, microRNA(miR)-134, an expression inhibitor of pluripotency markers including Oct4, Sox2 and Nanog, was decreased both in BPA-treated mESC and mEB. These results firstly indicate that BPA may disturb pluripotency in mESC and differentiation of mEB, and may inhibit ectodermal lineage differentiation of mEB while miR-134 may play a key role underlying this effect. (C) 2013 Elsevier Ltd. All rights reserved.
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收藏
页码：2249 / 2255
页数：7
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