The N-terminal region of two-pore channel 1 regulates trafficking and activation by NAADP

被引：21

作者：

Churamani, Dev ^{[1
]}

Hooper, Robert ^{[1
]}

Rahman, Taufiq ^{[2
]}

Brailoiu, Eugen ^{[3
]}

Patel, Sandip ^{[1
]}

机构：

[1] UCL, Dept Cell & Dev Biol, London WC1E 6BT, England

[2] Univ Cambridge, Dept Pharmacol, Cambridge CB2 1PD, England

[3] Temple Univ, Sch Med, Dept Pharmacol, Philadelphia, PA 19140 USA

来源：

BIOCHEMICAL JOURNAL | 2013年 / 453卷

基金：

英国生物技术与生命科学研究理事会;

关键词：

calcium; endosome; lysosome; nicotinic acid-adenine dinucleotide phosphate (NAADP); two-pore segment channel 1 (TPCN1); trafficking; ADENINE-DINUCLEOTIDE PHOSPHATE; MOBILIZES CALCIUM; CA2+; COORDINATION; ORGANELLES; AUTOPHAGY; PROTEINS; GRANULES; RELEASE; SODIUM;

D O I：

10.1042/BJ20130474

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

TPCs (two-pore channels) are NAADP (nicotinic acid-adenine dinucleotide phosphate)-sensitive Ca2+-permeable ion channels expressed on acidic organelles. In the present study we show that deletion of the N-terminal region redirects TPC1 to the ER (endoplasmic reticulum). The introduction of fluorophores at the N-terminus of TPC1 does not affect its subcellular location, but does reversibly abolish NAADP sensitivity. Our results reveal a dual role for the N-terminus in localization and function of TPC1.

引用

收藏

页码：147 / 151

页数：5

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