Zymosan promotes proliferation,Candida albicansadhesion and IL-1β production of oral squamous cell carcinoma in vitro

被引:13
作者
Chen, Xu [1 ]
Luo, Qingqiong [1 ]
Ding, Jieying [1 ]
Yang, Meng [1 ]
Zhang, Ruiyang [1 ]
Chen, Fuxiang [1 ,2 ]
机构
[1] Shanghai Jiao Tong Univ Sch Med SJTUSM, Shanghai Peoples Hosp 9, Dept Clin Immunol, Shanghai 200011, Peoples R China
[2] Shanghai Jiao Tong Univ Sch Med SJTUSM, Fac Med Lab Sci, Shanghai 200025, Peoples R China
基金
中国国家自然科学基金;
关键词
Zymosan; Candida albicans; Proliferation; Oral cancer; Oral squamous cell carcinoma; CANCER; CARCINOGENESIS; INFLAMMATION;
D O I
10.1186/s13027-020-00315-6
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Oral squamous cell carcinoma (OSCC) is the most common type of head and neck squamous cell carcinoma (HNSCC), and the effect of zymosan (ZYM), a component of the yeast cell wall, on oral cancer remains unclear. The CCK-8 proliferation assay was performed to evaluate the effect of ZYM on the proliferation of the OSCC cell lines WSU-HN4, WSU-HN6 and CAL27, and the potential mechanism was explored by quantitative real-time PCR, immunofluorescence assay and western blot. A cell adhesion assay was conducted to determine the adhesion ofCandida albicansto OSCC cells, and the expression of related genes, includingTLR2,MyD88,NLRP3,ASC,Caspase-1andIL-1 beta, and proteins, including TLR2, MyD88, NF-kappa B p65, p-NF-kappa B p65 and E-cadherin was determined. Additionally, the pro-inflammatory cytokines including IL-6, IL-8, TNF-alpha and IL-1 beta produced by OSCC cells were detected using a chemiluminescence immunoassay (CLIA). In the current study, the CCK-8 assay showed that ZYM promoted the proliferation of WSU-HN4, WSU-HN6 and CAL27 cells via the TLR2/MyD88 pathway. The cell adhesion assay showed that the number ofC. albicanscells per field significantly increased in ZYM-treated OSCC cells compared to controls. When treated with ZYM, OSCC cells secreted significantly more pro-inflammatory cytokine IL-1 beta, which could enhance inflammation in oral cancer microenvironment. In conclusion, ZYM from the fungal cell wall promotes the proliferation,C. albicansadhesion and IL-1 beta production in OSCC, as demonstrated by in vitro experiments.
引用
收藏
页数:8
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