DNA Origami Inside-Out Viruses

被引：34

作者：

Burns, Jonathan R. ^{[1
,2
]}

Lamarre, Baptiste ^{[1
]}

Pyne, Alice L. B. ^{[3
]}

Noble, James E. ^{[1
]}

Ryadnov, Maxim G. ^{[1
]}

机构：

[1] Natl Phys Lab, Hampton Rd, Teddington TW11 0LW, Middx, England

[2] UCL, Dept Chem, London WC1E 6BT, England

[3] UCL, London Ctr Nanotechnol, London WC1E 6BT, England

来源：

ACS SYNTHETIC BIOLOGY | 2018年 / 7卷 / 03期

基金：

英国工程与自然科学研究理事会;

关键词：

synthetic viruses; DNA origami; green fluorescent protein; transfection; microscopy; INTRACELLULAR DELIVERY; PEPTIDE; PROTEIN; NANOSTRUCTURES; ENCAPSULATION; RELEASE; DESIGN; SHAPES;

D O I：

10.1021/acssynbio.7b00278

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A synthetic topology for everted viruses is reported. The topology is a single-stranded virion DNA assembled into a hollow cube with exterior decorated with HIV-Tat transduction domains. The cube incorporates a pH responsive lid allowing for the controlled encapsulation of functional proteins and their transfer and release into live cells. Unlike viruses, which are protein shells with a [3,S]-fold rotational symmetry that encase nucleic acids, these cubes are [3, 4]-fold DNA boxes encapsulating proteins. Like viruses, such everted DNA-built viruses are monodisperse nanoscale assemblies that infect human cells with a specialist cargo. The design offers a bespoke bottom-up platform for engineering nonpolyhedral, nonprotein synthetic viruses.

引用

页码：767 / 773

页数：13

共 43 条

[1] Efficient intracellular delivery of a protein and a low molecular weight substance via recombinant polyomavirus-like particles [J].

Abbing, A ;

Blaschke, UK ;

Grein, S ;

Kretschmar, M ;

Stark, CMB ;

Thies, MJW ;

Walter, J ;

Weigand, M ;

Woith, DC ;

Hess, J ;

Reiser, COA .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (26) :27410-27421

[2] Self-assembly of a nanoscale DNA box with a controllable lid [J].

Andersen, Ebbe S. ;

Dong, Mingdong ;

Nielsen, Morten M. ;

Jahn, Kasper ;

Subramani, Ramesh ;

Mamdouh, Wael ;

Golas, Monika M. ;

Sander, Bjoern ;

Stark, Holger ;

Oliveira, Cristiano L. P. ;

Pedersen, Jan Skov ;

Birkedal, Victoria ;

Besenbacher, Flemming ;

Gothelf, Kurt V. ;

Kjems, Jorgen .

NATURE, 2009, 459 (7243) :73-U75

[3] Protein Coating of DNA Nanostructures for Enhanced Stability and Immunocompatibility [J].

Auvinen, Henni ;

Zhang, Hongbo ;

Nonappa ;

Kopilow, Alisa ;

Niemela, Elina H. ;

Nummelin, Sami ;

Correia, Alexandra ;

Santos, Helder A. ;

Linko, Veikko ;

Kostiainen, Mauri A. .

ADVANCED HEALTHCARE MATERIALS, 2017, 6 (18)

[4] Quantitative Packaging of Active Enzymes into a Protein Cage [J].

Azuma, Yusuke ;

Zschoche, Reinhard ;

Tinzl, Matthias ;

Hilvert, Donald .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2016, 55 (04) :1531-1534

[5] Accurate design of megadalton-scale two-component icosahedral protein complexes [J].

Bale, Jacob B. ;

Gonen, Shane ;

Liu, Yuxi ;

Sheffler, William ;

Ellis, Daniel ;

Thomas, Chantz ;

Cascio, Duilio ;

Yeates, Todd O. ;

Gonen, Tamir ;

King, Neil P. ;

Baker, David .

SCIENCE, 2016, 353 (6297) :389-394

[6] DNA rendering of polyhedral meshes at the nanoscale [J].

Benson, Erik ;

Mohammed, Abdulmelik ;

Gardell, Johan ;

Masich, Sergej ;

Czeizler, Eugen ;

Orponen, Pekka ;

Hogberg, Bjorn .

NATURE, 2015, 523 (7561) :441-U139

[7] A synthetic icosahedral DNA-based host-cargo complex for functional in vivo imaging [J].

Bhatia, Dhiraj ;

Surana, Sunaina ;

Chakraborty, Saikat ;

Koushika, Sandhya P. ;

Krishnan, Yamuna .

NATURE COMMUNICATIONS, 2011, 2

[8] VIPERdb2: an enhanced and web API enabled relational database for structural virology [J].

Carrillo-Tripp, Mauricio ;

Shepherd, Craig M. ;

Borelli, Ian A. ;

Venkataraman, Sangita ;

Lander, Gabriel ;

Natarajan, Padmaja ;

Johnson, John E. ;

Brooks, Charles L., III ;

Reddy, Vijay S. .

NUCLEIC ACIDS RESEARCH, 2009, 37 :D436-D442

[9] Structurally plastic peptide capsules for synthetic antimicrobial viruses [J].

Castelletto, Valeria ;

de Santis, Emiliana ;

Alkassem, Hasan ;

Lamarre, Baptiste ;

Noble, James E. ;

Ray, Santanu ;

Bella, Angelo ;

Burns, Jonathan R. ;

Hoogenboom, Bart W. ;

Ryadnov, Maxim G. .

CHEMICAL SCIENCE, 2016, 7 (03) :1707-1711

[10] Aptamer-Conjugated DNA Icosahedral Nanoparticles As a Carrier of Doxorubicin for Cancer Therapy [J].

Chang, Microsugar ;

Yang, Chung-Shi ;

Huang, Dong-Ming .

ACS NANO, 2011, 5 (08) :6156-6163

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