Spo0A regulates chromosome copy number during sporulation by directly binding to the origin of replication in Bacillus subtilis

被引：27

作者：

Boonstra, Mirjam ^{[1
]}

de Jong, Imke G. ^{[1
]}

Scholefield, Graham ^{[2
]}

Murray, Heath ^{[2
]}

Kuipers, Oscar P. ^{[1
,3
]}

Veening, Jan-Willem ^{[1
]}

机构：

[1] Univ Groningen, Mol Genet Grp, Groningen Biomol Sci & Biotechnol Inst, Ctr Synthet Biol, NL-9747 AG Groningen, Netherlands

[2] Newcastle Univ, Inst Cell & Mol Biosci, Ctr Bacterial Cell Biol, Newcastle Upon Tyne NE2 4AX, Tyne & Wear, England

[3] Kluyver Ctr Genom Ind Fermentat, Groningen, Netherlands

来源：

MOLECULAR MICROBIOLOGY | 2013年 / 87卷 / 04期

基金：

英国生物技术与生命科学研究理事会;

关键词：

DNA-REPLICATION; BACTERIAL SPORULATION; TRANSCRIPTION FACTOR; INITIATION PROTEIN; ESCHERICHIA-COLI; MASTER REGULATOR; GENE-EXPRESSION; SPORE FORMATION; SDA; INHIBITOR;

D O I：

10.1111/mmi.12141

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

When starved, Bacillus subtilis cells can enter the developmental programme of endospore formation by activation of the master transcriptional regulator Spo0A. Correct chromosome copy number is crucial for the production of mature and fully resistant spores. The production and maintenance of one chromosome for the mother cell and one copy for the forespore requires accurate co-ordination between DNA replication and initiation of sporulation. Here, we show that Spo0A regulates chromosome copy number by directly binding to a number of Spo0A binding sites that are present near the origin of replication (oriC). We demonstrate that cells lacking three specific Spo0A binding sites at oriC display increased chromosome copy numbers when sporulation is induced. Our data support the hypothesis that Spo0A directly controls DNA replication during sporulation by binding to oriC.

引用

页码：925 / 938

页数：14

共 43 条

[41] Nutritional control of elongation of DNA replication by (p)ppGpp [J].