Screening of microbes for lipases specific for saturated medium and long-chain fatty acids of milk fat

被引：10

作者：

Miettinen, Hanna ^{[1
]}

Nyyssola, Antti ^{[1
]}

Rokka, Susanna ^{[2
]}

Kontkanen, Hanna ^{[1
]}

Kruus, Kristiina ^{[1
]}

机构：

[1] VTT Biotechnol, FIN-02044 Espoo, Finland

[2] MTT Agrifood Res Finland, FI-31600 Jokioinen, Finland

来源：

INTERNATIONAL DAIRY JOURNAL | 2013年 / 32卷 / 02期

关键词：

CANDIDA-ANTARCTICA; SERUM-CHOLESTEROL; DIETARY-FAT; LIPIDS; PURIFICATION; CLONING; CANCER; MICE;

D O I：

10.1016/j.idairyj.2013.05.007

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Over 250 microbial strains were screened for lipase activity specific towards saturated medium and long-chain fatty acids. Strains showing trimyristin (C14:0) hydrolysis on agar medium were further streaked on tricaprylin (C8:0) and on olive oil (mostly C18:1) agar to identify strains hydrolysing C12-C15 fatty acids with modest or no activity towards short-chain and unsaturated fatty acids. The twenty strains showing the desired specificity were grown as liquid cultures and analysed for p-nitrophenyl (p-NP) myristate hydrolysis in the presence of Triton X-100. Cells from the cultures showing the highest activity towards p-NP myristate were analysed for specificity towards p-NP esters of different fatty acid chain lengths. On the basis of the p-NP ester profiling, four lipase samples were selected for hydrolysis experiments on butter oil. Of these, the sample obtained from the cultivation of Didpodascus capitatus met the screening criteria:C12:0-C16:0 specificity and no preference towards C18:0 and C18:1 fatty acids. (C) 2013 Published by Elsevier Ltd.

引用

页码：61 / 67

页数：7

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