Extended depth of field microscopy for rapid volumetric two-photon imaging

被引：103

作者：

Theriault, Gabrielle ^{[1
,2
]}

De Koninck, Yves ^{[1
,2
]}

McCarthy, Nathalie ^{[1
]}

机构：

[1] Univ Laval, COPL, Dept Phys Genie Phys & Opt, Quebec City, PQ G1V 0A6, Canada

[2] Ctr Rech Univ Laval Robert Giffard, Quebec City, PQ G1J 2G3, Canada

来源：

OPTICS EXPRESS | 2013年 / 21卷 / 08期

基金：

加拿大自然科学与工程研究理事会; 加拿大健康研究院;

关键词：

FLUORESCENCE MICROSCOPY; EXCITATION;

D O I：

10.1364/OE.21.010095

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Two-photon fluorescence microscopy is an influential tool in biology, providing valuable information on the activity of cells deep inside the tissue. However, it is limited by its low speed for imaging volume samples. Here we present the design of a two-photon scanning microscope with an extended and adjustable depth of field, which improves the temporal resolution for sampling thick samples. Moreover, this method implies no loss of optical power and resolution, and can be easily integrated into most commercial laser-scanning microscopy systems. We demonstrate experimentally the gain in performance of the system by comparing volumetric scans of neuronal structures with a standard versus an extended depth of field system. (c) 2013 Optical Society of America

引用

页码：10095 / 10104

页数：10

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