FRET-based aptamer biosensor for selective and sensitive detection of aflatoxin B1 in peanut and rice

被引：201

作者：

Sabet, Fereshte Sadat ^{[1
]}

Hosseini, Morteza ^{[1
,2
]}

Khabbaz, Hossein ^{[1
]}

Dadmehr, Mehdi ^{[3
]}

Ganjali, Mohammad Reza ^{[4
,5
]}

机构：

[1] Univ Tehran, Fac New Sci & Technol, Dept Life Sci Engn, Tehran, Iran

[2] Univ Tehran Med Sci, Med Biomat Res Ctr, Tehran, Iran

[3] Payame Noor Univ, Dept Biotechnol, Tehran, Iran

[4] Univ Tehran, Ctr Excellence Electrochem, Fac Chem, Tehran, Iran

[5] Univ Tehran Med Sci, Biosensor Res Ctr, Endocrinol & Metab Mol Cellular Sci Inst, Tehran, Iran

来源：

FOOD CHEMISTRY | 2017年 / 220卷

关键词：

Aflatoxin B1; Aptasensor; FRET; Sensitive detection; Fluorescence; Quantum dots; Nanobiosensor; Food; Au nanoparticle; IN-VITRO; B-1; APTASENSOR; LIGANDS;

D O I：

10.1016/j.foodchem.2016.10.004

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Aflatoxins are potential food pollutants produced by fungi. Among them, Aflatoxin B1 (AFB1) is the most toxic. Therefore, a great deal of concern is associated with AFB1 toxicity. In this work, utilizing a FRET-based method, we have developed a nanobiosensor for detection of AFB1 in agricultural foods. Aptamer-conjugated Quantum dots (QDs) are adsorbed to Au nanoparticles (AuNPs) due to interaction of aptamers with AuNPs leading to quenching effect on QDs fluorescence. Upon the addition of AFB1, the specific aptamers are attracted to AFB1, getting distance from AuNPs which result in fluorescence recovery. Under optimized conditions the detection limit of proposed nanobiosensor was 3.4 nM with linear range of 10-400 nM. Selectivity test demonstrates that the nanobiosensor could be a promising tool for specific evaluation of food stuff. This method was successfully applied for the analysis of AFB1 in rice and peanut samples. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：527 / 532

页数：6

共 24 条

[1] Semiconductor clusters, nanocrystals, and quantum dots [J].

Alivisatos, AP .

SCIENCE, 1996, 271 (5251) :933-937

[2] Aptamer-Based Analysis: A Promising Alternative for Food Safety Control [J].

Amaya-Gonzalez, Sonia ;

de-los-Santos-Alvarez, Noemi ;

Miranda-Ordieres, Arturo J. ;

Jesus Lobo-Castanon, Maria .

SENSORS, 2013, 13 (12) :16292-16311

[3] Determination of aflatoxins in hazelnuts by various sample preparation methods and liquid chromatography-tandem mass spectrometry [J].

Bacaloni, Alessandro ;

Cavaliere, Chiara ;

Cucci, Francesca ;

Foglia, Patrizia ;

Samperi, Roberto ;

Lagana, Aldo .

JOURNAL OF CHROMATOGRAPHY A, 2008, 1179 (02) :182-189

[4] Visual detection of cancer cells by colorimetric aptasensor based on aggregation of gold nanoparticles induced by DNA hybridization [J].

Borghei, Yasaman-Sadat ;

Hosseini, Morteza ;

Dadmehr, Mehdi ;

Hosseinkhani, Saman ;

Ganjali, Mohammad Reza ;

Sheikhnejad, Reza .

ANALYTICA CHIMICA ACTA, 2016, 904 :92-97

[5] INVITRO SELECTION OF RNA MOLECULES THAT BIND SPECIFIC LIGANDS [J].

ELLINGTON, AD ;

SZOSTAK, JW .

NATURE, 1990, 346 (6287) :818-822

[6]

Hosseini M, 2015, ACTA CHIM SLOV, V62, P721

[7] Aptamer in Bioanalytical Applications [J].

Iliuk, Anton B. ;

Hu, Lianghai ;

Tao, W. Andy .

ANALYTICAL CHEMISTRY, 2011, 83 (12) :4440-4452

[8] Determination of aflatoxins in animal feeds by HPLC with multifunctional column clean-up [J].

Khayoon, Wejdan Shakir ;

Saad, Bahruddin ;

Yan, Chew Bee ;

Hashim, Nor Hasani ;

Ali, Abdussalam Salhin Mohamed ;

Salleh, Muhammad Idiris ;

Salleh, Baharuddin .

FOOD CHEMISTRY, 2010, 118 (03) :882-886

[9] Aptamer-based nanobiosensors [J].

Kim, Yeon Seok ;

Raston, Nurul Hanun Ahmad ;

Gu, Man Bock .

BIOSENSORS & BIOELECTRONICS, 2016, 76 :2-19

[10] Gold nanoparticle-based homogeneous fluorescent aptasensor for multiplex detection [J].

Kim, Yeon Seok ;

Jurng, Jongsoo .

ANALYST, 2011, 136 (18) :3720-3724

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