Catalytic activities of fungal oxidases in hydrophobic ionic liquid 1-butyl-3-methylimidazolium hexafluorophosphate-based microemulsion

被引：46

作者：

Zhou, Gui-Ping ^{[1
]}

Zhang, Yun ^{[1
]}

Huang, Xi-Rong ^{[1
,2
]}

Shi, Chuan-Hong

Liu, Wei-Feng ^{[2
]}

Li, Yue-Zhong ^{[2
]}

Qu, Yin-Bo ^{[2
]}

Gao, Pei-Ji ^{[2
]}

机构：

[1] Shandong Univ, Educ Minist China, Key Lab Colloid & Interface Chem, Jinan 250100, Peoples R China

[2] Shandong Univ, State Key Lab Microbial Technol China, Jinan 250100, Peoples R China

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2008年 / 66卷 / 01期

基金：

中国国家自然科学基金;

关键词：

ionic liquids; microemulsion; oxidase; activity; viscosity;

D O I：

10.1016/j.colsurfb.2008.05.016

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

For hydrophobic ionic liquid 1-butyl-3-methylimidazolium hexafluorophosphate ([BMIM][PF6]), an H2O in-[BMIM][PF6] microemulsion could be formed in the presence of nonionic surfactant Triton X-100 (TX-100). In such a medium, both lignin peroxidase (LiP) and laccase could express their catalytic activity with the optimum molar ratio of H2O to TX-100 at 8.0 for LiP and > 20 for laccase, and the optimum pH values at 3.2 for LiP and 4.2 for laccase, respectively. As compared with pure or water saturated [BMIM][PF6], in which the two oxidases had negligible catalytic activity due to the strong inactivating effect of [BMIM][ PF6] on both enzymes, the use of the [BMIM][PF6]-based microemulsion had some advantages. Not only the catalytic activities of both fungal oxidases greatly enhanced, but also the apparent viscosity of the medium decreased. (c) 2008 Elsevier B.V. All rights reserved.

引用

页码：146 / 149

页数：4

共 28 条

[1] Enzymatic hydrolysis of microcrystalline cellulose in reverse micelles [J].