A study on permeability transition pore opening and cytochrome c release from mitochondria, induced by caspase-3 in vitro

被引:54
作者
Xia, T
Jiang, CS
Li, LJ
Wu, CH
Chen, Q [1 ]
Liu, SS
机构
[1] Chinese Acad Sci, Inst Zool, Natl Key Lab Biomembrane & Membrane Biotechnol, Beijing 100080, Peoples R China
[2] Peking Univ, Natl Key Lab Biomembrane & Membrane Biotectnol, Beijing 100871, Peoples R China
[3] Tianjin Inst Sports Med, Tianjin 300381, Peoples R China
关键词
apoptosis; mitochondrion; caspase-3; reactive oxygen species; permeability transition pore; cytochrome c;
D O I
10.1016/S0014-5793(01)03228-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We recently described that there is a feedback amplification of cytochrome c release from mitochondria by caspases. Here we investigated how caspases impact on mitochondria to induce cytochrome c release and found that recombinant caspase-3 induced opening of permeability transition pore and reduction of membrane potential in Nitro. These events were inhibited by Bcl-xL, cyclosporin A and z-VAD.fmk. Moreover, caspase-3 stimulated the rate of mitochondrial state 4 respiration, superoxide production and NAD(P)H oxidation in a Bcl-xL- and cyclosporin A-inhibitable manner. These results suggest that caspase-3 induces cytochrome c release by inducing permeability transition pore opening which is associated with changes in mitochondrial respiration and redox potential. (C) 2002 Published by Elsevier Science B.V. on be half of the Federation of European Biochemical Societies.
引用
收藏
页码:62 / 66
页数:5
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