A direct determination of thymidine triphosphate concentrations without dephosphorylation in peripheral blood mononuclear cells by LC/MS/MS

被引：11

作者：

Chi, JD ^{[1
]}

Jayewardene, A ^{[1
]}

Stone, J ^{[1
]}

Gambertoglio, JG ^{[1
]}

Aweeka, FT ^{[1
]}

机构：

[1] Univ Calif San Francisco, Sch Pharm, Drug Res Unit, Dept Clin Pharm, San Francisco, CA 94143 USA

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2001年 / 26卷 / 5-6期

关键词：

TTP; PBMC; LC/MS/MS system;

D O I：

10.1016/S0731-7085(01)00469-1

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A rapid, sensitive and specific analytical method with minimal sample preparation for the measurement of thymidine triphosphate (TTP) in peripheral blood mononuclear cells (PBMC) by LC/MS/MS has been developed. PBMC were separated from whole blood or buffy coat. The analyte and internal standard were extracted from PBMC with 70% methanol (pH 7.2). These extracts after centrifugation were directly injected onto LC/MS/MS without need for any further sample preparation. The calibration curve was linear over the range 0.8-800 ng/ml. Mean inter- and intra-assay coefficients of variation (CVs) over the range of the standard curve were less than 10%. The overall recovery of TTP was 103.5%. (C) 2001 Elsevier Science BN. All rights reserved.

引用

页码：829 / 836

页数：8

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