Cell-adhesive thermogelling PNIPAAm/hyaluronic acid cell delivery hydrogels for potential application as minimally invasive retinal therapeutics

被引：50
作者：
Mazumder, M. A. Jafar [1 ]
Fitzpatrick, Scott D. [2 ]
Muirhead, Ben [2 ]
Sheardown, Heather [1 ,2 ]
机构：
[1] McMaster Univ, Dept Chem Engn, Hamilton, ON L8S 4L7, Canada
[2] McMaster Univ, Sch Biomed Engn, Hamilton, ON L8S 4L7, Canada
来源：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A | 2012年 / 100A卷 / 07期
基金：
加拿大自然科学与工程研究理事会;
关键词：
thermally responsive; retina; cell delivery; poly(N-isopropylacrylamide); hyaluronic acid; HYALURONIC-ACID; BRUCHS MEMBRANE; TRANSPLANTATION; EPITHELIUM; ATTACHMENT; POLYMERS; COLLAGEN; CARRIERS;
D O I：
10.1002/jbm.a.34021
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
Copolymers of N-isopropylacrylamide (NIPAAm) and acrylic acid N-hydroxysuccinimide (NAS) were synthesized via free radical polymerization and conjugated with amine-functionalized hyaluronic acid (HA) and cell adhesive RGDS peptides. These novel copolymers were designed to facilitate noninvasive delivery of a liquid suspension of cells into the delicate subretinal space for treatment of retinal degenerative diseases such as age-related macular degeneration (AMD) and diabetic retinopathy. The various synthesized copolymers all displayed subphysiological phase transition temperatures, thereby allowing temperature-induced scaffold formation and subsequent entrapment of transplanted cells within an adhesive support matrix. Successful grafting of HA and RGDS peptides were confirmed with Fourier Transform Infrared (FTIR) spectroscopy and quantified with 1H Nuclear Magnetic Resonance (NMR) spectroscopy. All copolymers demonstrated excellent compatibility with retinal pigment epithelial (RPE) cells in culture and minimal host response was observed following subcutaneous implantation into hairless SKH1-E mice (strain code 447). (C) 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A 2012.
引用
收藏
页码：1877 / 1887
页数：11
相关论文
共 44 条
[1]   Long term treatment with sodium hyaluronate-containing artificial tears reduces ocular surface damage in patients with dry eye [J].
Aragona, P ;
Papa, V ;
Micali, A ;
Santocono, M ;
Milazzo, G .
BRITISH JOURNAL OF OPHTHALMOLOGY, 2002, 86 (02) :181-184
[2]   A hydrogel-based stem cell delivery system to treat retinal degenerative diseases [J].
Ballios, Brian G. ;
Cooke, Michael J. ;
van der Kooy, Derek ;
Shoichet, Molly S. .
BIOMATERIALS, 2010, 31 (09) :2555-2564
[3]   New hydrolysis-dependent thermosensitive polymer for an injectable degradable system [J].
Cui, Zhanwu ;
Lee, Bae Hoon ;
Vernon, Brent L. .
BIOMACROMOLECULES, 2007, 8 (04) :1280-1286
[4]   RPE transplantation and its role in retinal disease [J].
da Cruz, Lyndon ;
Chen, Fred K. ;
Ahmado, Ahmad ;
Greenwood, John ;
Coffey, Pete .
PROGRESS IN RETINAL AND EYE RESEARCH, 2007, 26 (06) :598-635
[5]  
Del Priore LV, 2002, CURR EYE RES, V25, P79
[6]   Corticosteroids inhibit VEGF-induced vascular leakage in a rabbit model of blood-retinal and blood-aqueous barrier breakdown [J].
Edelman, JL ;
Lutz, D ;
Castro, MR .
EXPERIMENTAL EYE RESEARCH, 2005, 80 (02) :249-258
[7]   Self-organizing optic-cup morphogenesis in three-dimensional culture [J].
Eiraku, Mototsugu ;
Takata, Nozomu ;
Ishibashi, Hiroki ;
Kawada, Masako ;
Sakakura, Eriko ;
Okuda, Satoru ;
Sekiguchi, Kiyotoshi ;
Adachi, Taiji ;
Sasai, Yoshiki .
NATURE, 2011, 472 (7341) :51-U73
[8]   PNIPAAm-Grafted-Collagen as an Injectable, In Situ Gelling, Bioactive Cell Delivery Scaffold [J].
Fitzpatrick, Scott D. ;
Mazumder, M. A. Jafar ;
Lasowski, Frances ;
Fitzpatrick, Lindsay E. ;
Sheardown, Heather .
BIOMACROMOLECULES, 2010, 11 (09) :2261-2267
[9]   HYALURONIC-ACID - A REVIEW OF ITS PHARMACOLOGY AND USE AS A SURGICAL AID IN OPHTHALMOLOGY, AND ITS THERAPEUTIC POTENTIAL IN JOINT DISEASE AND WOUND-HEALING [J].
GOA, KL ;
BENFIELD, P .
DRUGS, 1994, 47 (03) :536-566
[10]   TRANSPLANTATION OF CULTURED HUMAN RETINAL EPITHELIUM TO BRUCHS MEMBRANE OF THE OWL MONKEYS EYE [J].
GOURAS, P ;
FLOOD, MT ;
KJELDBYE, H ;
BILEK, MK ;
EGGERS, H .
CURRENT EYE RESEARCH, 1985, 4 (03) :253-265
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