Essential roles of 11β-HSD1 in regulating brown adipocyte function

被引：34

作者：

Liu, Juan ^{[1
]}

Kong, Xiaocen ^{[1
]}

Wang, Long ^{[1
]}

Qi, Hanmei ^{[1
]}

Di, Wenjuan ^{[1
]}

Zhang, Xiao ^{[3
]}

Wu, Lin ^{[1
]}

Chen, Xia ^{[1
]}

Yu, Jing ^{[1
]}

Zha, Juanmin ^{[1
]}

Lv, Shan ^{[1
]}

Zhang, Aisen ^{[1
]}

Cheng, Peng ^{[1
]}

Hu, Miao ^{[1
]}

Li, Yujie ^{[1
]}

Bi, Jianhua ^{[1
]}

Li, Yan ^{[5
]}

Hu, Fang ^{[5
]}

Zhong, Yi ^{[4
]}

Xu, Yong ^{[2
]}

Ding, Guoxian ^{[1
]}

机构：

[1] Nanjing Med Univ, Hosp 1, Dept Gerontol, Nanjing 210029, Jiangsu, Peoples R China

[2] Nanjing Med Univ, Dept Pathophysiol, Nanjing 210029, Jiangsu, Peoples R China

[3] Northwestern Univ, Feinberg Sch Med, Chicago, IL 60611 USA

[4] China Pharmaceut Univ, Dept Pharmaceut Chem, Nanjing, Jiangsu, Peoples R China

[5] Cent S Univ, Xiangya Hosp 2, Inst Metab & Endocrinol, Metab Syndrome Res Ctr, Changsha, Hunan, Peoples R China

来源：

JOURNAL OF MOLECULAR ENDOCRINOLOGY | 2013年 / 50卷 / 01期

基金：

中国国家自然科学基金;

关键词：

11 beta-hydroxysteroid dehydrogenase type 1; BVT.2733; brown adipose tissue; fatty beta-oxidation; obesity; 11-BETA-HYDROXYSTEROID DEHYDROGENASE TYPE-1; VISCERAL ADIPOSE-TISSUE; SELECTIVE-INHIBITION; INSULIN SENSITIVITY; METABOLIC SYNDROME; GENE-EXPRESSION; FAT; DIFFERENTIATION; OBESITY; OVEREXPRESSION;

D O I：

10.1530/JME-12-0099

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Brown adipose tissue (BAT) increases energy expenditure and is an attractive therapeutic target for obesity. 11 beta-Hydroxysteroid dehydrogenase type 1 (11 beta-HSD1), an amplifier of local glucocorticoid activity, has been shown to modulate white adipose tissue (WAT) metabolism and function. In this study, we investigated the roles of 11 beta-HSD1 in regulating BAT function. We observed a significant increase in the expression of BAT-specific genes, including UCP1, Cidea, Cox7a1, and Cox8b, in BVT.2733 (a selective inhibitor of 11 beta-HSD1)-treated and 11 beta-HSD1-deficient primary brown adipocytes of mice. By contrast, a remarkable decrease in BAT-specific gene expression was detected in brown adipocytes when 11 beta-HSD1 was overexpressed, which effect was reversed by BVT.2733 treatment. Consistent with the in vitro results, expression of a range of genes related to brown fat function in high-fat diet-fed mice treated with BVT.2733. Our results indicate that 11 beta-HSD1 acts as a vital regulator that controls the expression of genes related to brown fat function and as such may become a potential target in preventing obesity.

引用

页码：103 / 113

页数：11

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