Protective role of AQP3 in UVA-induced NHSFs apoptosis via Bcl2 up-regulation

被引：30

作者：

Xie, Hongfu ^{[1
]}

Liu, Fangfen ^{[1
]}

Liu, Leishan ^{[1
]}

Dan, Jian ^{[1
]}

Luo, Ying ^{[1
]}

Yi, Yuxin ^{[1
]}

Chen, Xiang ^{[1
]}

Li, Ji ^{[1
,2
,3
]}

机构：

[1] Cent S Univ, Xiangya Hosp, Dept Dermatol, Changsha 410008, Hunan, Peoples R China

[2] Cent S Univ, Xiangya Hosp, Ctr Mol Med, Changsha 410008, Hunan, Peoples R China

[3] Cent S Univ, Inst Med Genet, State Key Lab Med Genet, Changsha 410008, Hunan, Peoples R China

来源：

ARCHIVES OF DERMATOLOGICAL RESEARCH | 2013年 / 305卷 / 05期

基金：

高等学校博士学科点专项科研基金; 中国博士后科学基金; 中国国家自然科学基金;

关键词：

Aquaporin; 3; Apoptosis; UVA; Human skin fibroblasts; Bcl-2; INDUCED DOWN-REGULATION; HUMAN SKIN KERATINOCYTES; OXIDATIVE STRESS; CELL-DEATH; ULTRAVIOLET-RADIATION; CANCER CELLS; BETA-CELLS; AQUAPORIN-3; DISEASE; PROTEINS;

D O I：

10.1007/s00403-013-1324-y

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

Aquaporin-3 (AQP3), a water/glycerol-transporting protein that facilitates water, urea, and glycerol transport, can inhibit arsenite-induced apoptosis by up-regulating Bcl-2. However, whether it has a protective role in ultraviolet A (UVA)-induced apoptosis in normal human skin fibroblasts is not known. In this study, we demonstrate that mild UVA treatment fails to induce oxidative cell stress and apoptosis in normal human skin fibroblasts (NHSFs) overexpressing AQP3. After severe UVA irradiation, there was an increase in oxidative cell stress and apoptosis when AQP3 levels decreased. We also found that silencing AQP3 sensitized NHSFs to low-dose UVA. Overexpressing AQP3 was protective against high-dose UVA-induced oxidative stress and apoptosis. Besides, we observed that Bcl-2 may be involved in UVA-induced apoptosis. Our findings suggested that the water/glycerol-transporting protein AQP3 plays a role in resistance to UVA-induced apoptosis.

引用

页码：397 / 406

页数：10

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