Characterization of infectious hepatitis C virus from liver-derived cell lines

被引：9

作者：

Akazawa, Daisuke ^{[1
,2
]}

Date, Tomoko ^{[1
]}

Morikawa, Kenichi ^{[1
]}

Murayama, Asako ^{[1
]}

Omi, Noriaki ^{[1
,2
]}

Takahashi, Hitoshi ^{[1
,2
]}

Nakamura, Noriko ^{[2
]}

Ishii, Koji ^{[1
]}

Suzuki, Tetsuro ^{[1
]}

Mizokami, Masashi ^{[3
]}

Mochizuki, Hidenori ^{[2
]}

Wakita, Takaji ^{[1
]}

机构：

[1] Natl Inst Infect Dis, Dept Virol 2, Shinjuku Ku, Tokyo 1628640, Japan

[2] Toray Industries Ltd, Pharmaceut Res Labs, Kanagawa, Japan

[3] Nagoya City Univ, Grad Sch Med Sci, Dept Clin Mol Informat Med, Nagoya, Aichi, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 377卷 / 03期

基金：

日本学术振兴会;

关键词：

Cell culture; Hepatitis C virus; Infectivity; Particle; Replicon;

D O I：

10.1016/j.bbrc.2008.10.063

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The efficient production of infectious HCV from the JFH-1 strain is restricted to the Huh7 cell line and its derivatives. However, the factors involved in this restriction are unknown. In this study, we examined the production of infectious HCV from other liver-derived cell lines, and characterized the produced viruses. Clones of the Huh7, HepG2, and IMY-N9, harboring the JFH-1 full-genomic replicon, were obtained. The supernatant of each cell clone exhibited infectivity for naive Huh7. Each infectious supernatant was then characterized by sucrose density gradient. For all of the cell lines, the main peak of the HCV-core protein and RNA exhibited at approximately 1.15 g/mL of buoyant density. However, the supernatant from the IMY-N9 differed from that of Huh7 in the ratio of core:RNA at 1.15 g/mL and significant peaks were also observed at lower density. The virus particles produced from the different cell lines may have different characteristics. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：747 / 751

页数：5

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