Research on degradation of cellulose using enzyme treatment in flue-cured tobacco by 13C NMR spectroscopy

被引：11

作者：

Zhu, Xiaolan ^{[1
]}

Tan, Lanlan ^{[2
]}

Feng, Guanglin ^{[2
]}

Yang, Jun ^{[1
]}

Gao, Yun ^{[1
]}

Dai, Ya ^{[2
]}

机构：

[1] Univ Sci & Technol China, Res Ctr Tobacco & Hlth, Hefei 230052, Peoples R China

[2] China Tobacco Chuanyu Ind Corp, Ctr Technol, Chengdu 610066, Peoples R China

来源：

CELLULOSE | 2015年 / 22卷 / 04期

关键词：

Cellulose; Structure; Enzyme; Tobacco; Degradation; QUANTITATIVE-ANALYSIS; POLYSACCHARIDES; EXTRACTION; COMPONENTS; FIBERS; SUGARS;

D O I：

10.1007/s10570-015-0676-1

中图分类号：

TB3 [工程材料学]; TS [轻工业、手工业、生活服务业];

学科分类号：

0805 ; 080502 ; 0822 ;

摘要：

Cellulase (CE) and polygalacturonase (PG) were used to treat flue-cured tobacco, and then the content and structure of cellulose in tobacco were determined by C-13 NMR spectra. Neutral aroma substances in tobacco were also determined by the GC-MS method. The results showed that enzyme treatment could decompose cellulose and other cell wall substances in tobacco effectively, and the highest decrease degree for cellulose was 13.5 %. The crystalline allomorph analysis showed that enzyme treatment would lead to a decrease in the ratio of I-alpha and increase in I-beta; however, the paracrystalline ratio increased obviously after CE treatment but decreased after PG treatment. There was a significant increase in the amount of total neutral aroma substances, which increased by 38.7 % at a CE concentration of 20 g l(-1) compared to the control, while the PG treatment showed as much as that of the control.

引用

页码：2693 / 2702

页数：10

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