Fluorescent peptide displacement as a general assay for screening small molecule libraries against RNA

被引：29

作者：

Patwardhan, Neeraj N. ^{[1
]}

Cai, Zhengguo ^{[1
]}

Newson, Colby N. ^{[1
]}

Hargrove, Amanda E. ^{[1
]}

机构：

[1] Dept Chem, 124 Sci Dr,Box 90346, Durham, NC 27708 USA

来源：

ORGANIC & BIOMOLECULAR CHEMISTRY | 2019年 / 17卷 / 07期

关键词：

HIV-1 TAR RNA; BINDING SMALL MOLECULES; BIOACTIVE SMALL MOLECULES; SEQUENCE-BASED DESIGN; REV-RRE INTERACTION; AMINOGLYCOSIDE BINDING; DECODING REGION; A-SITE; DISCOVERY; RECOGNITION;

D O I：

10.1039/c8ob02467g

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

A prominent hurdle in developing small molecule probes against RNA is the relative scarcity of general screening methods. In this study, we demonstrate the application of a fluorescent peptide displacement assay to screen small molecule probes against four different RNA targets. The designed experimental protocol combined with statistical analysis provides a fast and convenient method to simultaneously evaluate small molecule libraries against different RNA targets and classify them based on affinity and selectivity patterns.

引用

页码：1778 / 1786

页数：9

共 50 条

[41] Use of solid supported nucleophiles and electrophiles for the purification of non-peptide small molecule libraries
Kaldor, SW
Siegel, MG
Fritz, JE
Dressman, BA
Hahn, PJ
TETRAHEDRON LETTERS, 1996, 37 (40) : 7193 - 7196
[42] Novel Aptamer-Based Small-Molecule Drug Screening Assay to Identify Potential Sclerostin Inhibitors against Osteoporosis
Lee, Chien-Ching
Hung, Chao-Ming
Chen, Chung-Hwan
Hsu, Yi-Chiang
Huang, Yuan-Pin
Huang, Tsung-Bin
Lee, Mon-Juan
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2021, 22 (15)
[43] Screening helix-threading peptides for RNA binding using a thiazole orange displacement assay
Krishnamurthy, Malathy
Schirle, Nicole T.
Beal, Peter A.
BIOORGANIC & MEDICINAL CHEMISTRY, 2008, 16 (19) : 8914 - 8921
[44] NEW PROMISE IN COMBINATORIAL CHEMISTRY - SYNTHESIS, CHARACTERIZATION, AND SCREENING OF SMALL-MOLECULE LIBRARIES IN SOLUTION
CARELL, T
WINTNER, EA
SUTHERLAND, AJ
REBEK, J
DUNAYEVSKIY, YM
VOUROS, P
CHEMISTRY & BIOLOGY, 1995, 2 (03): : 171 - 183
[45] Design of a small molecule that stimulates vascular endothelial growth factor A enabled by screening RNA fold-small molecule interactions
Haniff, Hafeez S.
Knerr, Laurent
Liu, Xiaohui
Crynen, Gogce
Bostrom, Jonas
Abegg, Daniel
Adibekian, Alexander
Lekah, Elizabeth
Wang, Kye Won
Cameron, Michael D.
Yildirim, Ilyas
Lemurell, Malin
Disney, Matthew D.
NATURE CHEMISTRY, 2020, 12 (10) : 952 - +
[46] A Peptide Microarray-Based Fluorescent and Resonance Light Scattering Assay for Screening Thrombin Inhibitor
Su Min
Li Tao
Liu Dian-Jun
Wang Zhen-Xin
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY, 2015, 43 (02) : 199 - 205
[47] SCAN™ - A High-Throughput Assay for Detecting Small Molecule Binding to RNA Targets
Baugh, Chris
Wang, Shaohui
Li, Bin
Appleman, James R.
Thompson, Peggy A.
JOURNAL OF BIOMOLECULAR SCREENING, 2009, 14 (03) : 219 - 229
[48] Cell-based approach for biosynthesis/screening cyclic peptide libraries against bacterial toxins
Camarero, J. A.
Kimura, R. H.
Tran, A. T.
JOURNAL OF PEPTIDE SCIENCE, 2006, 12 : 89 - 89
[49] A cell-based approach for the biosynthesis/screening of cyclic peptide libraries against bacterial toxins
Camarero, Julio A.
Kimura, Richard H.
Woo, Youn-Hi
Cantor, Jason
Steenblock, Erin
CHIMICA OGGI-CHEMISTRY TODAY, 2007, 25 (03) : 20 - 23
[50] Screening Assay for Small-Molecule Inhibitors of Synaptojanin 1, a Synaptic Phosphoinositide Phosphatase
McIntire, Laura Beth J.
Lee, Kyu-In
Chang-Ileto, Belle
Di Paolo, Gilbert
Kim, Tae-Wan
JOURNAL OF BIOMOLECULAR SCREENING, 2014, 19 (04) : 585 - 594

← 1 2 3 4 5 →