Immobilization of D-hydantoinase in polyaniline

被引：10

作者：

Andrade, Juliano M. ^{[1
]}

Peres, Rosa C. D. ^{[1
]}

Oesreicher, E. G. ^{[1
]}

Antunes, O. A. C. ^{[1
]}

Dariva, Claudio ^{[2
]}

机构：

[1] UFRJ, Inst Quim, Lab 641, CT, BR-21949900 Rio De Janeiro, Brazil

[2] Inst Pesquisa Tecnol ITP, Programa Mestrado Engn Proc UNIT, BR-49032490 Aracaju, SE, Brazil

来源：

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | 2008年 / 55卷 / 3-4期

关键词：

D-Hydantoinase; Immobilization process; Polyaniline; Adsorption; Amino acids;

D O I：

10.1016/j.molcatb.2008.01.011

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Polyaniline (PANI) is a water-in soluble polymer that has been used as support for enzyme immobilization due to its desirable characteristics, such as ease of preparation, high synthesis yield, high stability to temperature and pH, and resistance to microbial attack. In this work an investigation was carried out to determine the best conditions to immobilize D-hydantoinase (E.C. 3.5.2.2) in this support. As result, a simple and fast methodology for D-hydantoinase immobilization in PANI is described. 100% of proteins were immobilized on the support in concentrations up to 2 mg solid/ml. Higher concentrations led to a lower protein percentage immobilized. After five reaction cycles about a half of D-hydantoinase initial activity was conserved. (c) 2008 Elsevier B.V. All rights reserved.

引用

页码：185 / 188

页数：4

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