TISSUE DISTRIBUTION OF TRANSCRIPTS INVOLVED IN BIOSYNTHESIS OF BENZYLISOQUINOLINE ALKALOID IN MATURE PLANTS OF Argemone mexicana L. (Papaveraceae)

被引:0
作者
Vazquez-Flota, Felipe [1 ]
Rubio-Pina, Jorge [2 ]
Xool-Tamayo, Jorge [1 ,3 ]
Vergara-Olivares, Mariela [1 ]
Tamayo-Ordonez, Yahaira [1 ]
Monforte-Gonzalez, Miriam [1 ]
Guizar-Gonzalez, Cecilia
Miron-Lopez, Gumersindo [3 ]
机构
[1] Ctr Invest Cient Yucatan, Unidad Bioquim & Biol Mol Plantas, Calle 43,130 Chuburna, Merida 97205, Yucatan, Mexico
[2] Univ Autonoma Tlaxcala, Ctr Invest Genet & Ambiente, Km 10-5 Autopista Tlaxcala San Martin, Ixtacuixtla 90120, Tlaxcala, Mexico
[3] Univ Autonoma Yucatan, Fac Quim, Posgrad Interinst Ciencias Quim & Bioquim, Calle 43,613 X Calle 90, Merida 97069, Yucatan, Mexico
关键词
Argemone mexicana; benzylisoquinoline alkaloids; berberine; sanguinarine; CELL-CULTURES; YEAST EXTRACT; SANGUINARINE; POPPY; METABOLISM; EVOLUTION;
D O I
暂无
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
The distribution of berberine and sanguinarine was analyzed in roots, stems and leaves of mature Argemone mexicana plants, along with that of transcripts corresponding to selected genes involved in both early biosynthetic reactions, which are common to both alkaloids, and in the late specific reactions conducting to the formation of each of them. Roots were the main sites of alkaloid accumulation, though they showed the lowest accumulation of the analyzed transcripts. Results are discussed in terms of the operation of a possible transport mechanism of alkaloids between the aerial tissues and the roots, or the occurrence of different biosynthetic alternative reactions in both parts, aerial and underground tissues, involving different gene products, yet with similar catalytic capacities.
引用
收藏
页码:13 / 21
页数:9
相关论文
共 23 条
[1]   Argemone mexicana: chemical and pharmacological aspects [J].
Brahmachari, Goutam ;
Gorai, Dilip ;
Roy, Rajiv .
REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY, 2013, 23 (03) :559-575
[2]   Characterization of two methylenedioxy bridge-forming cytochrome P450-dependent enzymes of alkaloid formation in the Mexican prickly poppy Argemone mexicana [J].
Chavez, Maria Luisa Diaz ;
Rolf, Megan ;
Gesell, Andreas ;
Kutchan, Toni M. .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2011, 507 (01) :186-193
[3]   Transcript and metabolite profiling in cell cultures of 18 plant species that produce benzylisoquinoline alkaloids [J].
Farrow, Scott C. ;
Hagel, Jillian M. ;
Facchini, Peter J. .
PHYTOCHEMISTRY, 2012, 77 :79-88
[4]   Heterologous expression of two FAD-dependent oxidases with (S)-tetrahydroprotoberberine oxidase activity from Arge mone mexicana and Berberis wilsoniae in insect cells [J].
Gesell, Andreas ;
Diaz Chavez, Maria Luisa ;
Kramell, Robert ;
Piotrowski, Markus ;
Macheroux, Peter ;
Kutchan, Toni M. .
PLANTA, 2011, 233 (06) :1185-1197
[5]   Yeast extract induction of sanguinarine biosynthesis is partially dependent on the octadecanoic acid pathway in cell cultures of Argemone mexicana L., the Mexican poppy [J].
Guizar-Gonzalez, Cecilia ;
Monforte-Gonzalez, Miriam ;
Vazquez-Flota, Felipe .
BIOTECHNOLOGY LETTERS, 2016, 38 (07) :1237-1242
[6]  
Guízar-González C, 2012, J MEX CHEM SOC, V56, P19
[7]   Transcriptome analysis of 20 taxonomically related benzylisoquinoline alkaloid-producing plants [J].
Hagel, Jillian M. ;
Morris, Jeremy S. ;
Lee, Eun-Jeong ;
Desgagne-Penix, Isabel ;
Bross, Crystal D. ;
Chang, Limei ;
Chen, Xue ;
Farrow, Scott C. ;
Zhang, Ye ;
Soh, Jung ;
Sensen, Christoph W. ;
Facchini, Peter J. .
BMC PLANT BIOLOGY, 2015, 15
[8]   Benzylisoquinoline Alkaloid Metabolism: A Century of Discovery and a Brave New World [J].
Hagel, Jillian M. ;
Facchini, Peter J. .
PLANT AND CELL PHYSIOLOGY, 2013, 54 (05) :647-672
[9]   Characterization of a Flavoprotein Oxidase from Opium Poppy Catalyzing the Final Steps in Sanguinarine and Papaverine Biosynthesis [J].
Hagel, Jillian M. ;
Beaudoin, Guillaume A. W. ;
Fossati, Elena ;
Ekins, Andrew ;
Martin, Vincent J. J. ;
Facchini, Peter J. .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2012, 287 (51) :42972-42983
[10]  
Hernandez-Romero Y. M., 2005, Patent application, Patent No. [US 20150216181 A1, 20150216181]