Aberrant expression of Notch1/numb/snail signaling, an epithelial mesenchymal transition related pathway, in adenomyosis

被引：39
作者：
Qi, Shasha [1 ]
Zhao, Xingbo [1 ]
Li, Mingjiang [1 ]
Zhang, Xiaohui [1 ]
Lu, Zhenzhen [1 ]
Yang, Chunrun [1 ]
Zhang, Chunhua [1 ]
Zhang, Hui [1 ]
Zhang, Na [2 ]
机构：
[1] Shandong Univ, Shandong Prov Hosp, Dept Obstet & Gynecol, Jinan 250021, Shandong, Peoples R China
[2] Shandong Univ, Shandong Prov Hosp, Dept Anesthesiol & Surg, Jinan 250021, Shandong, Peoples R China
来源：
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY | 2015年 / 13卷
基金：
中国国家自然科学基金;
关键词：
Adenomyosis; Epithelial Mesenchymal Transition; Notch1/Numb/Snail Signaling; Slug; E-CADHERIN EXPRESSION; CELL-FATE; PROSTATE-CANCER; SNAIL; NUMB; TRANSCRIPTION; NOTCH1; EMT; MIGRATION; RECEPTOR;
D O I：
10.1186/s12958-015-0084-2
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Background: Epithelial mesenchymal transition (EMT) is involved in the pathogenesis of adenomyosis, and Notch signaling is crucial to EMT. The objective of this study was to explore Notch1/Numb/Snail signaling in adenomyosis. Methods: The expression levels of the members of the Notch1/Numb/Snail signaling cascade in normal endometria (proliferative phase: n = 15; secretory phase: n = 15; postmenopausal phase: n = 15) and adenomyotic endometria (proliferative phase: n = 15; secretory phase: n = 15) were determined by immunohistochemistry analysis. Results: We found that the expressions of Notch1 and the EMT-related proteins N-cadherin, Snail and Slug were upregulated in the ectopic endometrium of adenomyosis compared with normal endometrium. Numb, a negative regulator of Notch signaling, was significantly decreased in adenomyosis. In addition, reduced immunoexpression of E-cadherin was observed in adenomyosis. Conclusions: We conclude that Notch1/Numb/Snail signaling plays an important role in the pathogenesis and development of adenomyosis.
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页数：10
相关论文
共 57 条
[1]   Notch1 Is Regulated by Chorionic Gonadotropin and Progesterone in Endometrial Stromal Cells and Modulates Decidualization in Primates [J].
Afshar, Yalda ;
Miele, Lucio ;
Fazleabas, Asgerally T. .
ENDOCRINOLOGY, 2012, 153 (06) :2884-2896
[2]   Notch1 mediates uterine stromal differentiation and is critical for complete decidualization in the mouse [J].
Afshar, Yalda ;
Jeong, Jae-Wook ;
Roqueiro, Damian ;
DeMayo, Franco ;
Lydon, John ;
Radtke, Freddy ;
Radnor, Rachel ;
Miele, Lucio ;
Fazleabas, Asgerally .
FASEB JOURNAL, 2012, 26 (01) :282-294
[3]   RETRACTED: Notch-1 induces epithelial-mesenchymal transition consistent with cancer stem cell phenotype in pancreatic cancer cells (Retracted article. See vol. 423, pg. 153, 2018) [J].
Bao, Bin ;
Wang, Zhiwei ;
Ali, Shadan ;
Kong, Dejuan ;
Li, Yiwei ;
Ahmad, Aamir ;
Banerjee, Sanjeev ;
Azmi, Asfar S. ;
Miele, Lucio ;
Sarkar, Fazlul H. .
CANCER LETTERS, 2011, 307 (01) :26-36
[4]   The transcription factor Slug represses E-cadherin expression and induces epithelial to mesenchymal transitions:: a comparison with Snail and E47 repressors [J].
Bolós, V ;
Peinado, H ;
Pérez-Moreno, MA ;
Fraga, MF ;
Esteller, M ;
Cano, A .
JOURNAL OF CELL SCIENCE, 2003, 116 (03) :499-511
[5]   The transcription factor Snail controls epithelial-mesenchymal transitions by repressing E-cadherin expression [J].
Cano, A ;
Pérez-Moreno, MA ;
Rodrigo, I ;
Locascio, A ;
Blanco, MJ ;
del Barrio, MG ;
Portillo, F ;
Nieto, MA .
NATURE CELL BIOLOGY, 2000, 2 (02) :76-83
[6]   Roles for proteolysis and trafficking in notch maturation and signal transduction [J].
Chan, YM ;
Jan, YN .
CELL, 1998, 94 (04) :423-426
[7]  
Chang ACY, 2014, DEV DYNAM, V243, P894, DOI [10.1002/DVDY.24127, 10.1002/dvdy.24127]
[8]   Symmetric Division and Expression of Its Regulatory Gene Numb in Human Cervical Squamous Carcinoma Cells [J].
Chen, Huaizeng ;
Chen, Xiaojun ;
Ye, Feng ;
Lu, Weiguo ;
Xie, Xing .
PATHOBIOLOGY, 2009, 76 (03) :149-154
[9]   Hypoxia potentiates Notch signaling in breast cancer leading to decreased E-cadherin expression and increased cell migration and invasion [J].
Chen, J. ;
Imanaka, N. ;
Chen, J. ;
Griffin, J. D. .
BRITISH JOURNAL OF CANCER, 2010, 102 (02) :351-360
[10]   Oestrogen-induced epithelial-mesenchymal transition of endometrial epithelial cells contributes to the development of adenomyosis [J].
Chen, Yi-Jen ;
Li, Hsin-Yang ;
Huang, Chi-Hung ;
Twu, Nae-Fang ;
Yen, Ming-Shyen ;
Wang, Peng-Hui ;
Chou, Teh-Ying ;
Liu, Yen-Ni ;
Chao, Kuan-Chong ;
Yang, Muh-Hwa .
JOURNAL OF PATHOLOGY, 2010, 222 (03) :261-270
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