Activation of ERK/MAPK in the Lateral Amygdala of the Mouse is Required for Acquisition of a Fear-Potentiated Startle response

被引:24
作者
Di Benedetto, Barbara [1 ,2 ]
Kallnik, Magdalena [1 ]
Weisenhorn, Daniela M. Vogt [1 ,2 ]
Falls, William A. [3 ]
Wurst, Wolfgang [1 ,2 ]
Hoelter, Sabine M. [1 ]
机构
[1] Tech Univ Munich, Helmholtz Zentrum Munchen, Inst Dev Genet, German Res Ctr Environm Hlth, D-85764 Neuherberg, Bayern, Germany
[2] Max Planck Inst Psychiat, D-80804 Munich, Germany
[3] Univ Vermont, Dept Psychol, Burlington, VT 05405 USA
关键词
anxiety; amygdala; ERK/MAPK; fear-potentiated startle; mouse; LONG-TERM POTENTIATION; MESSENGER-RNA; MEMORY CONSOLIDATION; SYNAPTIC PLASTICITY; GLUTAMATE TRANSPORTERS; NERVOUS-SYSTEM; PROTEIN-KINASE; ANXIETY; MICE; BRAIN;
D O I
10.1038/npp.2008.57
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
There is considerable interest in examining the genes that may contribute to anxiety. We examined the function of ERK/MAPK in the acquisition of conditioned fear, as measured by fear-potentiated startle (FPS) in mice as a model for anticipatory anxiety in humans. We characterized the following for the first time in the mouse: ( 1) the expression of the ERK/MAPK signaling pathway components at the protein level in the lateral amygdala ( LA); ( 2) the time course of activation of phospho-activated MAPK in the LA after fear conditioning; ( 3) if pharmacological inhibition of pMAPK could modulate the acquisition of FPS; ( 4) the cell-type specificity of pMAPK in the LA after fear conditioning. Using western blot and immunohistochemistry techniques and injecting the MEK inhibitor U0126 in the LA, we showed the following: ( 1) both MEK1/MEK2 and ERK1/ERK2 were co-expressed in the LA of the adult mouse brain; ( 2) there is a peak of pMAPK at 60 min after fear conditioning; ( 3) the ERK/MAPK signaling pathway activation is essential for the acquisition of an FPS response; ( 4) at 60 min, the pMAPK are exclusively neuronal and not glial. These results emphasize the importance of this signaling pathway in the acquisition of conditioned fear in the mouse. Given the widely held view that conditioned fear models the essential aspects of anxiety disorders, the results confirm the ERK/MAPK signaling pathway as a molecular target for the treatment of anxiety disorders in the clinic.
引用
收藏
页码:356 / 366
页数:11
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