Frequency and dynamics of recombination within different species of human enteroviruses

被引:223
作者
Simmonds, P [1 ]
Welch, J [1 ]
机构
[1] Univ Edinburgh, Ctr Infect Dis, Virus Evolut Grp, Edinburgh EH9 1QH, Midlothian, Scotland
关键词
D O I
10.1128/JVI.80.1.483-493.2006
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Enteroviruses are members of the family Picornaviridae that cause widespread infections in human and other mammalian populations. Enteroviruses are genetically and antigenically highly variable, and recombination within and between serotypes contributes to their genetic diversity. To investigate the dynamics of the recombination process, sequence phylogenies between three regions of the genome (VP4, VP1, and 3Dpol) were compared among species A and B enterovirus variants detected in a human population-based survey in Scotland between 2000 and 2001, along with contemporary virus isolates collected in the same geographical region. This analysis used novel bioinformatic methods to quantify phylogenetic compatibility and correlations with serotype assignments of evolutionary trees constructed for different regions of the enterovirus genome. Species B enteroviruses showed much more frequent, time-correlated recombination events than those found for species A, despite the equivalence in population sampling, concordant with a linkage analysis of previously characterized enterovirus sequences obtained over longer collection periods. An analysis of recombination among complete genome sequences by computation of a phyllogenetic compatibility matrix (PCM) demonstrated sharply defined boundaries between the VP2/VP3/VP1 block and sequences to either side in phylogenetic compatibility. The PCM also revealed equivalent or frequently greater degrees of incompatibility between different parts within the nonstructural region (2A-3D), indicating the occurrence of extensive recombination events in the past evolution of this part of the genome. Together, these findings provide new insights into the dynamics of species A and B enterovirus recombination and evolution and into the contribution of structured sampling to documenting reservoirs, emergence, and spread of novel recombinant forms in human populations.
引用
收藏
页码:483 / 493
页数:11
相关论文
共 44 条
  • [1] Molecular analysis of the echovirus 18 prototype - Evidence of interserotypic recombination with echovirus 9
    Andersson, P
    Edman, K
    Lindberg, AM
    [J]. VIRUS RESEARCH, 2002, 85 (01) : 71 - 83
  • [2] BODIAN MD, 1965, VITAL RICKETTSIAL IN, P430
  • [3] Molecular epidemiology and evolution of enterovirus 71 strains isolated from 1970 to 1998
    Brown, BA
    Oberste, MS
    Alexander, JP
    Kennett, ML
    Pallansch, MA
    [J]. JOURNAL OF VIROLOGY, 1999, 73 (12) : 9969 - 9975
  • [4] ANTIGEN CHIMERAS OF POLIOVIRUS AS POTENTIAL NEW VACCINES
    BURKE, KL
    DUNN, G
    FERGUSON, M
    MINOR, PD
    ALMOND, JW
    [J]. NATURE, 1988, 332 (6159) : 81 - 82
  • [5] Multiple sequence alignment with the Clustal series of programs
    Chenna, R
    Sugawara, H
    Koike, T
    Lopez, R
    Gibson, TJ
    Higgins, DG
    Thompson, JD
    [J]. NUCLEIC ACIDS RESEARCH, 2003, 31 (13) : 3497 - 3500
  • [6] Molecular comparison of echovirus 11 strains circulating in Europe during an epidemic of multisystem hemorrhagic disease of infants indicates that evolution generally occurs by recombination
    Chevaliez, S
    Szendröi, A
    Caro, V
    Balanant, J
    Guillot, S
    Berencsi, G
    Delpeyroux, F
    [J]. VIROLOGY, 2004, 325 (01) : 56 - 70
  • [7] Comparison of the complete nucleotide sequences of echovirus 7 strain UMMC and the prototype (Wallace) strain demonstrates significant genetic drift over time
    Chua, BH
    McMinn, PC
    Lam, SK
    Chua, KB
    [J]. JOURNAL OF GENERAL VIROLOGY, 2001, 82 : 2629 - 2639
  • [8] Genomic features of intertypic recombinant Sabin poliovirus strains excreted by primary vaccinees
    Cuervo, NS
    Guillot, S
    Romanenkova, N
    Combiescu, M
    Aubert-Combiescu, A
    Seghier, M
    Caro, V
    Crainic, R
    Delpeyroux, F
    [J]. JOURNAL OF VIROLOGY, 2001, 75 (13) : 5740 - 5751
  • [9] Nonreplicative homologous RNA recombination: Promiscuous joining of RNA pieces?
    Gmyl, AP
    Korshenko, SA
    Belousov, EV
    Khitrina, EV
    Agol, VI
    [J]. RNA, 2003, 9 (10) : 1221 - 1231
  • [10] Nonreplicative RNA recombination in poliovirus
    Gmyl, AP
    Belousov, EV
    Maslova, SV
    Khitrina, EV
    Chetverin, AB
    Agol, VI
    [J]. JOURNAL OF VIROLOGY, 1999, 73 (11) : 8958 - 8965