Immunohistochemical analysis of matrix metalloproteinase-13 in human caries dentin

被引：22
作者：
Loreto, C. [1 ]
Galanti, C. [2 ]
Musumeci, G. [1 ]
Rusu, M. C. [3 ]
Leonardi, R. [2 ]
机构：
[1] Univ Catania, Human Anat & Histol Sect, Dept Biomed Sci, I-95123 Catania, Italy
[2] Univ Catania, Dent Unit 2, Dept Med & Surg Sci, I-95123 Catania, Italy
[3] Carol Davila Univ Med & Pharm, Fac Med Dent, Discipline Anat, Bucharest, Romania
来源：
EUROPEAN JOURNAL OF HISTOCHEMISTRY | 2014年 / 58卷 / 01期
关键词：
Human teeth; caries; MMP-13; dentin; immunohistochemistry;
D O I：
10.4081/ejh.2014.2318
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The immunoexpression profile of matrix metalloproteinase-13 was investigated for the first time in dentin of human caries and healthy teeth. Twelve permanent premolars (10 caries and 2 sound) were decalcified in ethylenediaminetetraacetic acid and proces sed for embedding in paraffin wax. Sections 34 mu m in thickness were cut and processed for immunohistochemistry. A mouse monoclonal anti-metalloproteinase-13 antibody was used for localisation using an immunoperoxidase technique. Dentinal immunoreactivity was detected in all teeth; it was weak in sound teeth and strong close to the caries area. These in vivo findings suggest a role for metalloproteinase-13 in the development and progression of adult human dental tissue disorders.
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收藏
页码：47 / 51
页数：5
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