The Francisella tularensis proteome and its recognition by antibodies

被引:17
作者
Kilmury, Sara L. N. [1 ]
Twine, Susan M. [1 ]
机构
[1] Natl Res Council Canada, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada
来源
FRONTIERS IN MICROBIOLOGY | 2011年 / 2卷
关键词
Francisella tularensis; LVS; immunoproteomics; proteome microarray; 2D-PAGE; antibody; vaccine; mouse vaccination; LIVE VACCINE STRAIN; CELL-MEDIATED-IMMUNITY; OUTER-MEMBRANE PROTEIN; INTRACELLULAR BACTERIAL PATHOGEN; PROTECTIVE IMMUNITY; HUMORAL IMMUNITY; IMMUNOREACTIVE ANTIGENS; PULMONARY CHALLENGE; HELICOBACTER-PYLORI; VIRULENT-STRAINS;
D O I
10.3389/fmicb.2010.00143
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Francisella tularensis is the causative agent of a spectrum of diseases collectively known as tularemia. The extreme virulence of the pathogen in humans, combined with the low infectious dose and the ease of dissemination by aerosol have led to concerns about its abuse as a bioweapon. Until recently, nothing was known about the virulence mechanisms and even now, there is still a relatively poor understanding of pathogen virulence. Completion of increasing numbers of Francisella genome sequences, combined with comparative genomics and proteomics studies, are contributing to the knowledge in this area. Tularemia may be treated with antibiotics, but there is currently no licensed vaccine. An attenuated strain, the Live Vaccine Strain (LVS) has been used to vaccinate military and at risk laboratory personnel, but safety concerns mean that it is unlikely to be licensed by the FDA for general use. Little is known about the protective immunity induced by vaccination with LVS, in humans or animal models. Immunoproteomics studies with sera from infected humans or vaccinated mouse strains, are being used in gel-based or proteome microarray approaches to give insight into the humoral immune response. In addition, these data have the potential to be exploited in the identification of new diagnostic or protective antigens, the design of next generation live vaccine strains, and the development of subunit vaccines. Herein, we briefly review the current knowledge from Francisella comparative proteomics studies and then focus upon the findings from immunoproteomics approaches.
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页数:22
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共 138 条
[1]   TLR4-mediated activation of dendritic cells by the heat shock protein DnaK from Francisella tularensis [J].
Ashtekar, Amit R. ;
Zhang, Ping ;
Katz, Jannet ;
Deivanayagam, Champion C. S. ;
Rallabhandi, Prasad ;
Vogel, Stefanie N. ;
Michalek, Suzanne M. .
JOURNAL OF LEUKOCYTE BIOLOGY, 2008, 84 (06) :1434-1446
[2]   Multimethodological Approach to Identification of Glycoproteins from the Proteome of Francisella tularensis, an Intracellular Microorganism [J].
Balonova, Lucie ;
Hernychova, Lenka ;
Mann, Benjamin F. ;
Link, Marek ;
Bilkova, Zuzana ;
Novotny, Milos V. ;
Stulik, Jiri .
JOURNAL OF PROTEOME RESEARCH, 2010, 9 (04) :1995-2005
[3]   The role of pilin glycan in neisserial pathogenesis [J].
Banerjee, A ;
Ghosh, SK .
MOLECULAR AND CELLULAR BIOCHEMISTRY, 2003, 253 (1-2) :179-190
[4]   Robust Salmonella metabolism limits possibilities for new antimicrobials [J].
Becker, D ;
Selbach, M ;
Rollenhagen, C ;
Ballmaier, M ;
Meyer, TF ;
Mann, M ;
Bumann, D .
NATURE, 2006, 440 (7082) :303-307
[5]   VIRULENCE OF BACTERIUM-TULARENSE .1. A STUDY OF THE VIRULENCE OF BACTERIUM-TULARENSE IN MICE, GUINEA PIGS, AND RABBITS [J].
BELL, JF ;
OWEN, CR ;
LARSON, CL .
JOURNAL OF INFECTIOUS DISEASES, 1955, 97 (02) :162-166
[6]   AGGLUTININS AND ANTIBODIES TO FRANCISELLA-TULARENSIS OUTER-MEMBRANE ANTIGENS IN THE EARLY DIAGNOSIS OF DISEASE DURING AN OUTBREAK OF TULAREMIA [J].
BEVANGER, L ;
MAELAND, JA ;
NAESS, AI .
JOURNAL OF CLINICAL MICROBIOLOGY, 1988, 26 (03) :433-437
[7]   Active suppression of the pulmonary immune response by Francisella tularensis Schu4 [J].
Bosio, Catharine M. ;
Bielefeldt-Ohmann, Helle ;
Belisle, John T. .
JOURNAL OF IMMUNOLOGY, 2007, 178 (07) :4538-4547
[8]   ENZYME-LINKED IMMUNOSORBENT ASSAY FOR IMMUNOLOGICAL DIAGNOSIS OF HUMAN TULAREMIA [J].
CARLSSON, HE ;
LINDBERG, AA ;
LINDBERG, G ;
HEDERSTEDT, B ;
KARLSSON, KA ;
AGELL, BO .
JOURNAL OF CLINICAL MICROBIOLOGY, 1979, 10 (05) :615-621
[9]   Type IV pili in Francisella tularensis:: Roles of pilF and pilT in fiber assembly, host cell adherence, and virulence [J].
Chakraborty, Subhra ;
Monfett, Michael ;
Maier, Tamara M. ;
Benach, Jorge L. ;
Frank, Dara W. ;
Thanassi, David G. .
INFECTION AND IMMUNITY, 2008, 76 (07) :2852-2861
[10]   Comparative Genomic Characterization of Francisella tularensis Strains Belonging to Low and High Virulence Subspecies [J].
Champion, Mia D. ;
Zeng, Qiandong ;
Nix, Eli B. ;
Nano, Francis E. ;
Keim, Paul ;
Kodira, Chinnappa D. ;
Borowsky, Mark ;
Young, Sarah ;
Koehrsen, Michael ;
Engels, Reinhard ;
Pearson, Matthew ;
Howarth, Clint ;
Larson, Lisa ;
White, Jared ;
Alvarado, Lucia ;
Forsman, Mats ;
Bearden, Scott W. ;
Sjoestedt, Anders ;
Titball, Richard ;
Michell, Stephen L. ;
Birren, Bruce ;
Galagan, James .
PLOS PATHOGENS, 2009, 5 (05)