Preparation and antibacterial activity of lysozyme and layered double hydroxide nanocomposites

被引：47
作者：
Yang, Qin-Zheng [1 ,2 ]
Chang, Ying-Yue [1 ,3 ]
Zhao, Hua-Zhang [1 ,3 ]
机构：
[1] Peking Univ, Dept Environm Engn, Beijing 100871, Peoples R China
[2] Shandong Polytech Univ, Coll Food & Bioengn, Shandong Prov Key Lab Microbial Engn, Jinan 250353, Peoples R China
[3] Minist Educ, Key Lab Water & Sediment Sci, Beijing 100871, Peoples R China
来源：
WATER RESEARCH | 2013年 / 47卷 / 17期
基金：
中国国家自然科学基金;
关键词：
Lysozyme; Layered double hydroxide; Intercalation; Disinfection; Water purification; CONTROLLED-RELEASE; IMMOBILIZATION; BACTERIA; REMOVAL; ENZYME; FUNCTIONALIZATION; INTERCALATION; NANOMATERIALS; DISINFECTION; HYDROTALCITE;
D O I：
10.1016/j.watres.2013.09.002
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
It is necessary to develop "green" disinfection technology which does not produce disinfection by-products. Lysozyme-layered double hydroxide nanocomposites (LYZ-LDHs) were prepared by intercalating LYZ in LDH for the first time. Their antibacterial activity was evaluated using staphylococcus aureus as a target. The bacteria removal mechanism was also studied. Characterization of LYZ-LDHs by X-ray diffraction and Fourier transform infrared spectroscopy indicated that LYZ was successfully intercalated in LDH, compressed and deformed without secondary structural change. LYZ-LDHs showed excellent bactericidal effectiveness against staphylococcus aureus. The antibacterial performance of LYZ-LDHs was found to be affected by the LYZ/LDH ratio and the pH of the bacteria-containing water. The bacteria removal efficiency of LYZ-LDHs with LYZ/LDH mass ratio of 0.8 was consistently above 94% over the pH range of 3-9. LYZ-LDHs adsorbed bacteria to their surface by LDH and then killed them by the immobilized LYZ. This new material integrated the bactericidal ability of LYZ and adsorption ability of LDH. Moreover, the antibacterial ability of LYZ-LDHs was persistent and not limited by the adsorption capacity. (C) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码：6712 / 6718
页数：7
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