Synchronous fluorescence analysis of phytate in food

被引：22

作者：

Chen, Yingyu ^{[1
]}

Chen, Jingwen ^{[2
]}

Luo, Zuowen ^{[1
]}

Ma, Kang ^{[1
]}

Chen, Xiaoqing ^{[1
]}

机构：

[1] Cent S Univ, Sch Chem & Chem Engn, Changsha 410083, Peoples R China

[2] Yancheng Inst Technol, Sch Chem & Biol Engn, Yancheng 224003, Peoples R China

来源：

MICROCHIMICA ACTA | 2009年 / 164卷 / 1-2期

基金：

中国国家自然科学基金;

关键词：

Phytate; Synchronous fluorescence; Complex reaction; 1; 10-Phenanthroline; PERFORMANCE LIQUID-CHROMATOGRAPHY; PHYTIC ACID; COMPLEX-FORMATION; AQUEOUS-SOLUTION; EXCHANGE; ION; 1,10-PHENANTHROLINE; DEGRADATION; BINDING; ZINC;

D O I：

10.1007/s00604-008-0026-1

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel synchronous fluorescence method is described for determination of phytic acid in food samples. It is based on the formation of a ternary complex between phytate, 1,10-phenanthroline (phen) and Fe3+. The synchronous fluorescence intensity of the solution was accordingly enhanced proportionally to the increased phytate concentration. Synchronous luminescence spectroscopy was adopted in the study, and the Delta lambda was set to 40 nm. The calibration graph is linear from 0.33 to 32 mg L-1 with a linear equation of I (f) = 8.770 + 2.980c (R (2) > 0.9994). The method was applied to determine phytate in food samples and the found concentrations of phytic acid in food were in the range of 4.62-24.08 mg g(-1) with recoveries of 92.2%-98.3%. The control experiments were performed using UV-spectrophotometry method, and the results showed the method to be reliable.

引用

页码：35 / 40

页数：6

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