Staphylococcus aureus Membrane-Derived Vesicles Promote Bacterial Virulence and Confer Protective Immunity in Murine Infection Models

被引：44

作者：

Askarian, Fatemeh ^{[1
,2
]}

Lapek, John D., Jr. ^{[3
]}

Dongre, Mitesh ^{[4
]}

Tsai, Chih-Ming ^{[2
]}

Kumaraswamy, Monika ^{[5
]}

Kousha, Armin ^{[1
,2
]}

Valderrama, J. Andres ^{[2
]}

Ludviksen, Judith A. ^{[6
]}

Cavanagh, Jorunn P. ^{[7
,8
]}

Uchiyama, Satoshi ^{[2
]}

Mollnes, Tom E. ^{[6
,9
,10
,11
,12
]}

Gonzalez, David J. ^{[3
,13
]}

Wai, Sun N. ^{[4
]}

Nizet, Victor ^{[2
,13
]}

Johannessen, Mona ^{[1
]}

机构：

[1] UiT Arctic Univ Norway, Fac Hlth Sci, Dept Med Biol, Res Grp Host Microbe Interact, Tromso, Norway

[2] Univ Calif San Diego, Dept Pediat, Div Host Microbe Syst & Therapeut, La Jolla, CA 92093 USA

[3] Univ Calif San Diego, Dept Pharmacol, La Jolla, CA 92093 USA

[4] Umea Univ, Dept Mol Biol, Lab Mol Infect Med Sweden, Umea, Sweden

[5] Univ Calif San Diego, Dept Med, Div Infect Dis, La Jolla, CA 92093 USA

[6] Nordland Hosp, Res Lab, Bodo, Norway

[7] Univ Hosp North Norway, Dept Pediat & Neonatal Med, Tromso, Norway

[8] UiT Arctic Univ Norway, Fac Hlth Sci, Dept Pediat, Tromso, Norway

[9] UiT Arctic Univ Norway, KG Jebsen Thrombosis Res & Expertise Ctr, Fac Hlth Sci, Tromso, Norway

[10] Univ Oslo, Oslo Univ Hosp, Dept Immunol, Oslo, Norway

[11] Univ Oslo, KG Jebsen Inflammat Res Ctr, Oslo, Norway

[12] Norwegian Univ Sci & Technol, Ctr Mol Inflammat Res, Trondheim, Norway

[13] Univ Calif San Diego, Skaggs Sch Pharm & Pharmaceut Sci, La Jolla, CA 92093 USA

来源：

FRONTIERS IN MICROBIOLOGY | 2018年 / 9卷

基金：

瑞典研究理事会; 美国国家卫生研究院;

关键词：

Staphylococcus aureus; membrane-derived vesicles; proteomics; systemic infection; protective immunity; NEUTROPHIL EXTRACELLULAR TRAPS; GRAM-POSITIVE BACTERIA; CELL-DEATH; SKIN INFLAMMATION; MASS-SPECTROMETRY; NEGATIVE BACTERIA; ATOPIC-DERMATITIS; BIOGENESIS; HOST; MECHANISM;

D O I：

10.3389/fmicb.2018.00262

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Staphylococcus aureus produces membrane-derived vesicles (MVs), which share functional properties to outer membrane vesicles. Atomic force microscopy revealed that S. aureus-derived MVs are associated with the bacterial surface or released into the surrounding environment depending on bacterial growth conditions. By using a comparative proteomic approach, a total of 131 and 617 proteins were identified in MVs isolated from S. aureus grown in Luria-Bertani and brain-heart infusion broth, respectively. Purified S. aureus MVs derived from the bacteria grown in either media induced comparable levels of cytotoxicity and neutrophil-activation. Administration of exogenous MVs increased the resistance of S. aureus to killing by whole blood or purified human neutrophils ex vivo and increased S. aureus survival in vivo. Finally, immunization of mice with S. aureus-derived MVs induced production of IgM, total IgG, IgG1, IgG2a, and IgG2b resulting in protection against subcutaneous and systemic S. aureus infection. Collectively, our results suggest S. aureus MVs can influence bacterial-host interactions during systemic infections and provide protective immunity in murine models of infection.

引用

页数：17

共 71 条

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