Pyrosequencing reveals an oseltamivir-resistant marker in the quasispecies of avian influenza A (H7N9) virus

被引：5
|
作者：
Mok, Chee-Keng [1 ,2 ,3 ]
Chang, Shih-Cheng [1 ,2 ]
Chen, Guang-Wu [1 ,2 ,4 ]
Lo, Yu-Lun [1 ,2 ]
Chen, Shu-Jen [3 ,5 ]
Wu, Ho-Sheng [6 ]
Liu, Ming-Tsan [6 ]
Chang, Feng-Yee [6 ]
Lin, Tzou-Yien [7 ]
Shih, Shin-Ru [1 ,2 ,3 ]
机构：
[1] Chang Gung Univ, Res Ctr Emerging Viral Infect, Taoyuan 333, Taiwan
[2] Chang Gung Mem Hosp, Taiwan Ctr Dis Control Collaborating Lab Viro, Clin Virol Lab, Taoyuan, Taiwan
[3] Chang Gung Univ, Coll Med, Grad Inst Biomed Sci, Taoyuan 333, Taiwan
[4] Chang Gung Univ, Coll Engn, Dept Comp Sci & Informat Engn, Taoyuan 333, Taiwan
[5] Chang Gung Univ, Mol Med Res Ctr, Taoyuan 333, Taiwan
[6] Ctr Dis Control, Res & Diagnost Ctr, Taipei, Taiwan
[7] Minist Hlth & Welf, Taipei, Taiwan
来源：
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION | 2015年 / 48卷 / 04期
关键词：
Avian influenza A virus; H7N9; Oseltamivir-resistant marker; Pyrosequencing; NEURAMINIDASE INHIBITOR RESISTANCE; SURVEILLANCE; INFECTION;
D O I：
10.1016/j.jmii.2013.09.010
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Prompt diagnosis of an oseltamivir-resistant marker is important for patient management, in particular to prevent the spread of resistant strains in the recent human H7N9 outbreak. We tailored a pyrosequencing assay to reveal neuraminidase R292K, a resistant marker found in one isolate from China, and demonstrated its performance in both sensitivity and specificity. In addition, a semi-nested polymerase chain reaction was applied, which enhanced the detection rate by at least 10-fold. We validated this assay by examining the marker in Taiwan's first imported human case and found R and K in quasispecies. Copyright (C) 2013, Taiwan Society of Microbiology. Published by Elsevier Taiwan LLC. All rights reserved.
引用
收藏
页码：465 / 469
页数：5
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