Denitration of L-type calcium channel

被引：23

作者：

Kang, Minho ^{[1
]}

Akbarali, Hamid I. ^{[1
]}

机构：

[1] Virginia Commonwealth Univ, Dept Pharmacol & Toxicol, Richmond, VA 23298 USA

来源：

FEBS LETTERS | 2008年 / 582卷 / 20期

关键词：

nitric oxide; tyrosine; nitration; ion channel; src kinase; smooth muscle;

D O I：

10.1016/j.febslet.2008.07.042

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Tyrosine nitration results in altered function of selective proteins, including human smooth muscle L-type calcium channel, hCa(v)1.2b. We report here that Ca(v)1.2 is also subject to "denitration''. Cell lysates from activated macrophage-like cell line, RAW264.7 cells, reversed peroxynitrite-induced nitration of the carboxy terminus of Cav1.2 in a 1D gel assay. Tyrosine phosphorylation of the calcium channel by c-src kinase was blocked by nitration but reversed by pretreatment with RAW264.7 cell lysates. These findings indicate that denitration may be a physiological mechanism to restore cellular excitability during inflammation. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：3033 / 3036

页数：4

共 17 条

[1] Gastrointestinal motility disorders in patients with inactive Crohn's disease [J].

Annese, V ;

Bassotti, G ;

Napolitano, G ;

Usai, P ;

Andriulli, A ;

Vantrappen, G .

SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY, 1997, 32 (11) :1107-1117

[2] APPARENT HYDROXYL RADICAL PRODUCTION BY PEROXYNITRITE - IMPLICATIONS FOR ENDOTHELIAL INJURY FROM NITRIC-OXIDE AND SUPEROXIDE [J].

BECKMAN, JS ;

BECKMAN, TW ;

CHEN, J ;

MARSHALL, PA ;

FREEMAN, BA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (04) :1620-1624

[3] Nitric oxide (NO) - Biogeneration, regulation, and relevence to human diseases [J].

Bian, K ;

Murad, F .

FRONTIERS IN BIOSCIENCE-LANDMARK, 2003, 8 :D264-D278

[4] Reversible inhibition of mammalian glutamine synthetase by tyrosine nitration [J].

Goerg, Boris ;

Qvartskhava, Natalia ;

Voss, Peter ;

Grune, Tilman ;

Haeussinger, Dieter ;

Schliess, Freimut .

FEBS LETTERS, 2007, 581 (01) :84-90

[5] Modulation of voltage-dependent Ca2+ channels in rabbit colonic smooth muscle cells by c-Src and focal adhesion kinase [J].

Hu, XQ ;

Singh, N ;

Mukhopadhyay, D ;

Akbarali, HI .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (09) :5337-5342

[6] Histone H1.2 is a substrate for denitrase, an activity that reduces nitrotyrosine immunoreactivity in proteins [J].

Irie, Y ;

Saeki, M ;

Kamisaki, Y ;

Martin, E ;

Murad, F .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (10) :5634-5639

[7] PEROXYNITRITE-MEDIATED TYROSINE NITRATION CATALYZED BY SUPEROXIDE-DISMUTASE [J].

ISCHIROPOULOS, H ;

ZHU, L ;

CHEN, J ;

TSAI, M ;

MARTIN, JC ;

SMITH, CD ;

BECKMAN, JS .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1992, 298 (02) :431-437

[8] An activity in rat tissues that modifies nitrotyrosine-containing proteins [J].

Kamisaki, Y ;

Wada, K ;

Bian, K ;

Balabanli, B ;

Davis, K ;

Martin, E ;

Behbod, F ;

Lee, YC ;

Murad, F .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (20) :11584-11589

[9] COOH-terminal association of human smooth muscle calcium channel Cav1.2b with Src kinase protein binding domains:: effect of nitrotyrosylation [J].

Kang, Minho ;

Ross, Gracious R. ;

Akbarali, Hamid I. .

AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2007, 293 (06) :C1983-C1990

[10] Peroxynitrite disables the tyrosine phosphorylation regulatory mechanism: Lymphocyte-specific tyrosine kinase fails to phosphorylate nitrated cdc2(6-20)NH2 peptide [J].

Kong, SK ;

Yim, MB ;

Stadtman, ER ;

Chock, PB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (08) :3377-3382

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